Graptemys kerneri, n. sp., from the Suwannee River drainage of north-central Florida, represents the most southeastern occurrence of the genus. This species is morphologically and geographically most similar to the extant Barbour's map turtle, Graptemys barbouri. G. kerneri exhibits sexual dimorphism similar to extant G. barbouri, G. ernsti, G. pulchra, and G. gibbonsi, with females being megacephalic and attaining a much larger size than males. The new species possesses a very wide skull and mandible, making it the most blunt-headed member of its clade. Specimens described here include a nearly complete skull, eight mandibles, two epiplastra, 34 neural bones, and an assortment of other shell fragments. Previously reported fossil material from Florida was collected in the 1960s along the Santa Fe River and referred to both the Pliocene and Pleistocene. Rare earth element (REE) analysis of this material is reinterpreted here as being Rancholabrean in age.