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1 October 1986 IMMUNOBLOT ASSAY: A RAPID AND SENSITIVE METHOD FOR IDENTIFICATION OF SALMONID FISH VIRUSES
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Abstract

An immunoblot assay was used to identify the viruses of infectious pancreatic necrosis, infectious hematopoietic necrosis, and viral hemorrhagic septicemia. Viral antigen in infected cell culture supernatant was adsorbed onto nitrocellulose membrane or Whatman 541 filter paper and detected by enzyme-linked immunosorbent assay techniques. The immunoblot assay took less than 4 hr to perform and required no special instrumentation. Assays using cell culture supernatant fluids showed immunoblot sensitivity was 105–106 PFU/ml. Assay sensitivity, determined using purified virus, is 0.85–4.0 ng of viral antigen. The immunoblot assay was used to detect and identify virus in cell culture fluids.

McAllister and Schill: IMMUNOBLOT ASSAY: A RAPID AND SENSITIVE METHOD FOR IDENTIFICATION OF SALMONID FISH VIRUSES
P. E. McAllister and W. B. Schill "IMMUNOBLOT ASSAY: A RAPID AND SENSITIVE METHOD FOR IDENTIFICATION OF SALMONID FISH VIRUSES," Journal of Wildlife Diseases 22(4), 468-474, (1 October 1986). https://doi.org/10.7589/0090-3558-22.4.468
Received: 1 October 1985; Published: 1 October 1986
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