Serum samples were collected from 116 wolves which were captured in southcentral Alaska during 1975 through 1982. Antibodies to the following infectious disease agents were found: infectious canine hepatitis virus—72 of 87 (81%), canine parvovirus type 2—0 of 55 (0%) through 1979 and 10 of 32 (31%) after 1979, Francisella tularensis— 16 of 67 (25%), canine distemper virus—10 of 83 (12%), Coxiella burnetti—5 of 95 (5%), rabies virus—1 of 88 (1%), Brucella spp.—1 of 67 (1%), Leptospira interrogans—1 of 82 (1%). Apparently rabies, brucellosis, and leptospirosis were rare and had little effect on the wolf population. Conversely, the other five infections were comparatively common and may have had a negative impact on the health of specific individual wolves, but did not appear to influence the health of the population.