Infections caused by a Sphaerospora sp. resembling S. chinensis are reported for the first time in goldfish (Carassius auratus) from North America. The myxosporean was found in the respiratory epithelium of the gill of pond-reared fish. Spores from stained tissue sections were spherical with an equal mean length and width of 6.3 μm. Spore valves were thickened at the suture which lies in a plane perpendicular to two prominent pyriform polar capsules. The polar capsules were 4.0 × 2.8 μm in length and width. Both monosporous and disporous development within a surrounding “pseudoplasmodium” was detected. Infections caused moderate hyperplasia and occasional necrosis of the respiratory epithelial cells of the gill.