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1 October 1997 Extracting Protostrongylus spp. Larvae from Bighorn Sheep Feces
Sean G. Forrester, Murray W. Lankester
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Abstract

First-stage larvae of Protostrongylus spp. were more numerous in the core of bighorn sheep (Ovis canadiensis canadiensis) pellets than near the surface. As a result, only 22% could be extracted from whole pellets and the numbers collected did not reflect the total number of larvae present in samples. Crushing semi-dried pellets yielded seven times as many larvae and numbers collected were correlated with totals present. The use of tissue, in addition to a screen filter in a beaker extraction method, produced a cleaner sample and did not affect larval collection or the correlation. By comparison, most first-stage larvae of Parelaphostrongylus tenuis from white-tailed deer (Odocoileus virginianus) were near the surface of fecal pellets where they may be removed readily by water.

Sean G. Forrester and Murray W. Lankester "Extracting Protostrongylus spp. Larvae from Bighorn Sheep Feces," Journal of Wildlife Diseases 33(4), 868-872, (1 October 1997). https://doi.org/10.7589/0090-3558-33.4.868
Received: 6 April 1997; Published: 1 October 1997
KEYWORDS
Beaker method
extracting nematode larvae
fecal examination
Protostrongylus rushi
Protostrongylus stilesi
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