A Case of Pulmonary Cryptococcosis in a Free-living Toad (Bufo bufo)

Fernanda Seixas; Maria da Luz Martins; Maria de Lurdes Pinto; Paulo Jorge Travassos; Manuela Miranda; Maria dos Anjos Pires

doi:10.7589/0090-3558-44.2.460

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1 April 2008 A Case of Pulmonary Cryptococcosis in a Free-living Toad (Bufo bufo)

Fernanda Seixas, Maria da Luz Martins, Maria de Lurdes Pinto, Paulo Jorge Travassos, Manuela Miranda, Maria dos Anjos Pires

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J. of Wildlife Diseases, 44(2):460-463 (2008). https://doi.org/10.7589/0090-3558-44.2.460

Abstract

Pulmonary cryptococcosis was observed in a free-living adult female common toad (Bufo bufo) that was killed by a vehicle. Both lungs had various eosinophilic, monomorphic, and spherical to elliptical organisms identified as Cryptoccocus spp. The yeasts were demonstrated by Grocott's silver method and the periodic acid-Schiff reaction and the capsule was positive for mucin with a mucicarmine stain. The agent was confirmed by immunohistochemistry, using the monoclonal antibody anti-Cryptococcus neoformans, and by a polymerase chain reaction-based method using a C. neoformans-specific primer. This report, to the best of our knowledge, represents the first case of cryptococcosis in a common toad.

Seixas, da Luz Martins, de Lurdes Pinto, Travassos, Miranda, and dos Anjos Pires: A Case of Pulmonary Cryptococcosis in a Free-living Toad (Bufo bufo)

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Fernanda Seixas, Maria da Luz Martins, Maria de Lurdes Pinto, Paulo Jorge Travassos, Manuela Miranda, and Maria dos Anjos Pires "A Case of Pulmonary Cryptococcosis in a Free-living Toad (Bufo bufo)," Journal of Wildlife Diseases 44(2), 460-463, (1 April 2008). https://doi.org/10.7589/0090-3558-44.2.460

Received: 14 June 2006; Published: 1 April 2008

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