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1 July 2008 Bighorn Sheep β2-Integrin LFA-1 Serves as a Receptor for Mannheimia haemolytica Leukotoxin
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Abstract

Mannheimia haemolytica is an important cause of pneumonia in bighorn sheep (BHS; Ovis canadensis). Leukotoxin (Lkt), the primary virulence determinant of M. haemolytica, induces cytolysis of all subsets of leukocytes. Previously, we have shown that CD18, the β subunit of β2-integrins, mediates Lkt-induced cytolysis. However, it is not clear whether CD18 of all three β2-integrins, LFA-1, Mac-1, and CR4, mediates Lkt-induced cytol-ysis. The objective of this study was to determine whether BHS LFA-1 (CD11a/CD18) serves as a receptor for Lkt. Plasmids encoding cDNA for BHS CD11a and CD18 were cotransfected into Lkt-resistant HEK-293 cells. Flow cytometric analysis of transfectants confirmed cell surface expression of BHS LFA-1, Lkt-LFA-1 binding and Lkt-induced intracellular calcium elevation. More importantly, the transfectants were efficiently lysed by Lkt in a concentration-dependent manner. Collectively, these results indicate that BHS LFA-1 serves as a functional receptor for M. haemolytica Lkt.

Dassanayake, Liu, Davis, Foreyt, and Srikumaran: Bighorn Sheep β2-Integrin LFA-1 Serves as a Receptor for Mannheimia haemolytica Leukotoxin
Rohana P. Dassanayake, Weiguo Liu, William C. Davis, William J. Foreyt, and Subramaniam Srikumaran "Bighorn Sheep β2-Integrin LFA-1 Serves as a Receptor for Mannheimia haemolytica Leukotoxin," Journal of Wildlife Diseases 44(3), 743-747, (1 July 2008). https://doi.org/10.7589/0090-3558-44.3.743
Received: 12 April 2007; Published: 1 July 2008
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