Eleven species of wild waterfowl (Anseriformes and Charadriiformes) were found dead in the Hongjian Nur Lake in the Ordos wetland, Inner Mongolia of northern China, in 2007. Pasteurella multocida was isolated from tissue samples of dead and sick birds and identified as P. multocida subsp. multocida, serotype A1, using serologic and molecular techniques. Eight bird species in this outbreak had never been previously reported with P. m. multocida infection. This was also the first report of fowl cholera in wild waterfowl in China.