PREVALENCE OF ANTIBODIES TO TYPE A INFLUENZA VIRUS IN WILD AVIAN SPECIES USING TWO SEROLOGIC ASSAYS

Justin D. Brown; M. Page Luttrell; Roy D. Berghaus; Whitney Kistler; Shamus P. Keeler; Andrea Howey; Benjamin Wilcox; Jeffrey Hall; Larry Niles; Amanda Dey; Gregory Knutsen; Kristin Fritz; David E. Stallknecht

doi:10.7589/0090-3558-46.3.896

Translator Disclaimer

You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.

Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.

1 July 2010 PREVALENCE OF ANTIBODIES TO TYPE A INFLUENZA VIRUS IN WILD AVIAN SPECIES USING TWO SEROLOGIC ASSAYS

Author Affiliations +

Justin D. Brown, M. Page Luttrell, Roy D. Berghaus, Whitney Kistler, Shamus P. Keeler, Andrea Howey, Benjamin Wilcox
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA
Jeffrey Hall
US Geological Survey, National Wildlife Health Center, Madison, Wisconsin 53711, USA
Larry Niles
Conserve Wildlife Foundation of New Jersey, PO Box 400, Trenton, New Jersey 08625, USA
Amanda Dey
Endangered and Nongame Species Program, New Jersey Division of Fish and Wildlife, Assunpink Wildlife Management Area, 1 Eldridge Road, Upper Freehold Township, Robbinsville, New Jersey 08691, USA
Gregory Knutsen, Kristin Fritz
Agassiz National Wildlife Refuge, Middle River, Minnesota 56737, USA
David E. Stallknecht
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA

J. of Wildlife Diseases, 46(3):896-911 (2010). https://doi.org/10.7589/0090-3558-46.3.896

ARTICLE
CITED BY
DOWNLOAD PDF SAVE TO MY LIBRARY

PERSONAL SIGN IN
Full access may be available with your subscription

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account
or Access Institutional Sign In via Shibboleth or OpenAthens

PURCHASE THIS CONTENT

PURCHASE SINGLE ARTICLE

Price: $20.00 ADD TO CART

Includes PDF & HTML, when available

RIGHTS & PERMISSIONS

Get copyright permission

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Abstract

Serologic testing to detect antibodies to avian influenza (AI) virus has been an underused tool for the study of these viruses in wild bird populations, which traditionally has relied on virus isolation and reverse transcriptase-polymerase chain reaction (RT-PCR). In a preliminary study, a recently developed commercial blocking enzyme-linked immunosorbent assay (bELISA) had sensitivity and specificity estimates of 82% and 100%, respectively, for detection of antibodies to AI virus in multiple wild bird species after experimental infection. To further evaluate the efficacy of this commercial bELISA and the agar gel immunodiffusion (AGID) test for AI virus antibody detection in wild birds, we tested 2,249 serum samples collected from 62 wild bird species, representing 10 taxonomic orders. Overall, the bELISA detected 25.4% positive samples, whereas the AGID test detected 14.8%. At the species level, the bELISA detected as many or more positive serum samples than the AGID in all 62 avian species. The majority of positive samples, detected by both assays, were from species that use aquatic habitats, with the highest prevalence from species in the orders Anseriformes and Charadriiformes. Conversely, antibodies to AI virus were rarely detected in the terrestrial species. The serologic data yielded by both assays are consistent with the known epidemiology of AI virus in wild birds and published reports of host range based on virus isolation and RT-PCR. The results of this research are also consistent with the aforementioned study, which evaluated the performance of the bELISA and AGID test on experimental samples. Collectively, the data from these two studies indicate that the bELISA is a more sensitive serologic assay than the AGID test for detecting prior exposure to AI virus in wild birds. Based on these results, the bELISA is a reliable species-independent assay with potentially valuable applications for wild bird AI surveillance.

Citation Download Citation

Justin D. Brown, M. Page Luttrell, Roy D. Berghaus, Whitney Kistler, Shamus P. Keeler, Andrea Howey, Benjamin Wilcox, Jeffrey Hall, Larry Niles, Amanda Dey, Gregory Knutsen, Kristin Fritz and David E. Stallknecht "PREVALENCE OF ANTIBODIES TO TYPE A INFLUENZA VIRUS IN WILD AVIAN SPECIES USING TWO SEROLOGIC ASSAYS," Journal of Wildlife Diseases 46(3), (1 July 2010). https://doi.org/10.7589/0090-3558-46.3.896

Received: 26 September 2009; Accepted: 1 January 2010; Published: 1 July 2010

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account
or Access Institutional Sign In via Shibboleth or OpenAthens

PURCHASE THIS CONTENT

PURCHASE SINGLE ARTICLE

Price: $20.00 ADD TO CART

Includes PDF & HTML, when available