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1 July 2010 Detection of Hypoderma actaeon Infestation in Cervus elaphus with ELISA and Western Blotting
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Abstract

We used enzyme-linked immunosorbent assay (ELISA) and western blotting (WB) to evaluate the reactivity of first-stage larval extracts of Hypoderma lineatum with antibodies in sera from 76 red deer from an endemic area for Hypoderma actaeon. Antibodies in sera from deer infested with H. actaeon recognized hypodermin C from H. lineatum in both ELISA and WB assays. ELISA values were correlated with the epidemiology of the fly infestation in the area where the deer sera were collected. There was no clear relationship between anti-hypodermin C antibody levels and the age of the animals or the number of Hypoderma grubs found at necropsy in the hides of the deer. Our results suggest that first-instar antigens of H. lineatum can be used to diagnose natural infestations by H. actaeon by indicating the presence of first-stage larvae, which can help to accurately describe H. actaeon epidemiology.

Julia Domínguez, Rosario Panadero, and Concepción de la Fuente-López "Detection of Hypoderma actaeon Infestation in Cervus elaphus with ELISA and Western Blotting," Journal of Wildlife Diseases 46(3), 934-938, (1 July 2010). https://doi.org/10.7589/0090-3558-46.3.934
Received: 20 July 2009; Accepted: 1 December 2009; Published: 1 July 2010
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