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1 April 2012 Use of Real-time PCR to Detect Canine Parvovirus in Feces of Free-ranging Wolves
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Abstract

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 104 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

L. David Mech, Emily S. Almberg, Douglas Smith, Sagar Goyal, and Randall S. Singer "Use of Real-time PCR to Detect Canine Parvovirus in Feces of Free-ranging Wolves," Journal of Wildlife Diseases 48(2), 473-476, (1 April 2012). https://doi.org/10.7589/0090-3558-48.2.473
Received: 9 May 2011; Accepted: 1 November 2011; Published: 1 April 2012
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