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1 January 2014 Utility of a Fecal Real-time PCR Protocol for Detection of Mycobacterium bovis Infection in African Buffalo (Syncerus caffer)
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Abstract
A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis–infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette–Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.
© 2014 Wildlife Disease Association
Annette Roug, Claire Geoghegan, Elizabeth Wellington, Woutrina A. Miller, Emma Travis, David Porter, David Cooper, Deana L. Clifford, Jonna A. K. Mazet and Sven Parsons "Utility of a Fecal Real-time PCR Protocol for Detection of Mycobacterium bovis Infection in African Buffalo (Syncerus caffer)," Journal of Wildlife Diseases 50(1), (1 January 2014). https://doi.org/10.7589/2013-07-159
Received: 10 July 2013; Accepted: 1 July 2013; Published: 1 January 2014
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