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1 January 2015 Multiplex Serology for Common Viral Infections in Feral Pigs (Sus scrofa) in Hawaii between 2007 and 2010
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Abstract

Multiplex serology was performed for the detection of total immunoglobulin (Ig) and IgM antibodies against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and swine influenza virus (SIV) antigens in feral swine (Sus scrofa). Serum samples were collected from the islands of Oahu (292 pigs) and Hawaii (52 pigs) between 2007 and 2010. The highest antibody prevalence was to PCV2 (63%), followed by SIV (7.8%) and PRRSV (5.8%). Antigen-specific IgM was detected at a much lower prevalence. PCR amplification and sequence analysis of PCV2 in three IgM-positive samples identified PCV2b as the only genotype. While the prevalence of PCV2 and PRRSV remained similar between 2007 and 2010, the percentage of SIV-positive samples on Oahu increased from 2% to 19%. Our results demonstrate the utility of multiplex serology for pathogen surveillance in feral pig populations.

Wildlife Disease Association 2015
Rachel J. Stephenson, Benjamin R. Trible, Yu Wang, Maureen A. Kerrigan, Samuel M. Goldstein, and Raymond R. R. Rowland "Multiplex Serology for Common Viral Infections in Feral Pigs (Sus scrofa) in Hawaii between 2007 and 2010," Journal of Wildlife Diseases 51(1), 239-243, (1 January 2015). https://doi.org/10.7589/2013-09-242
Received: 13 September 2013; Accepted: 1 June 2014; Published: 1 January 2015
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