Translator Disclaimer
1 April 2016 RANAVIRUS CAUSES MASS DIE-OFFS OF ALPINE AMPHIBIANS IN THE SOUTHWESTERN ALPS, FRANCE
Author Affiliations +
Abstract

Pathogenic fungi and viruses cause mortality outbreaks in wild amphibians worldwide. In the summer of 2012, dead tadpoles and adults of the European common frog Rana temporaria were reported in alpine lakes in the southwestern Alps (Mercantour National Park, France). A preliminary investigation using molecular diagnostic techniques identified a Ranavirus as the potential pathogenic agent. Three mortality events were recorded in the park, and samples were collected. The amphibian chytrid fungus Batrachochytrium dendrobatidis was not detected in any of the dead adult and juvenile frogs sampled (n=16) whereas all specimens were positive for a Ranavirus. The genome sequence of this Ranavirus was identical to previously published sequences of the common midwife toad virus (CMTV), a Ranavirus that has been associated with amphibian mortalities throughout Europe. We cultured virus from the organs of the dead common frogs and infecting adult male common frogs collected in another alpine region where no frog mortality had been observed. The experimentally infected frogs suffered 100% mortality (n=10). The alpine die-off is the first CMTV outbreak associated with mass mortality in wild amphibians in France. We describe the lesions observed and summarize amphibian populations affected by Ranaviruses in Europe. In addition, we discuss the ecologic specificities of mountain amphibians that may contribute to increasing their risk of exposure to and transmission of Ranaviruses.

Claude Miaud, Françoise Pozet, Nadine Curt Grand Gaudin, An Martel, Frank Pasmans, and Sophie Labrut "RANAVIRUS CAUSES MASS DIE-OFFS OF ALPINE AMPHIBIANS IN THE SOUTHWESTERN ALPS, FRANCE," Journal of Wildlife Diseases 52(2), 242-252, (1 April 2016). https://doi.org/10.7589/2015-05-113
Received: 4 May 2015; Accepted: 1 October 2015; Published: 1 April 2016
JOURNAL ARTICLE
11 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top