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1 July 2016 EFFECT OF STORAGE TIME AND STORAGE CONDITIONS ON ANTIBODY DETECTION IN BLOOD SAMPLES COLLECTED ON FILTER PAPER
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Abstract

Using filter paper to collect blood from wildlife for antibody analysis can be a powerful technique to simplify the collection, transport, and storage of blood samples. Despite these advantages, there are limited data that detail how long these samples can be stored and how storage conditions affect antibody longevity. We used blood samples collected on filter paper from coyotes experimentally infected with Yersinia pestis to determine optimum sample storage conditions over time. Blood samples collected on filter paper were stored for 454 d or more in four groups: 1) at ambient temperature and at ambient relative humidity, 2) at ambient temperature with desiccant, 3) at 4 C with desiccant, and 4) at −20 C with desiccant. Samples stored at 4 C or −20 C with desiccant had detectable antibody for a longer period of time than the samples stored at room temperature.

Sarah Bevins, Ryan Pappert, John Young, Brandon Schmit, Dennis Kohler, and Laurie Baeten "EFFECT OF STORAGE TIME AND STORAGE CONDITIONS ON ANTIBODY DETECTION IN BLOOD SAMPLES COLLECTED ON FILTER PAPER," Journal of Wildlife Diseases 52(3), 478-483, (1 July 2016). https://doi.org/10.7589/2015-09-242
Received: 15 September 2015; Accepted: 1 December 2015; Published: 1 July 2016
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