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1 January 2019 Morphologic Observations and Novel 18S rRNA Sequences of Abbreviata hastaspicula and Abbreviata antarctica from Varanus spp. Lizards in Australia
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Abstract

The nematodes Abbreviata antarctica von Linstow, 1899, and Abbreviata hastaspiculaJones, 1979, are predominant spirurid nematodes in species of Varanus lizards in Australia. However, genetic knowledge of these two species of nematode is lacking. In this study, nematodes removed from Varanus gouldii were examined using integrated morphologic and molecular methods. We extracted DNA from A. hastaspicula and A. antarctica for PCR and sequencing. Specific 18S small subunit ribosomal RNA (rRNA) primers were designed on the basis of existing 18S rRNA sequences of Physalopterinae strains. Species of Abbreviata, which are closely similar morphologically, may be misidentified, especially the larvae of different species of Abbreviata that cannot be differentiated. The findings of this study will improve the accuracy in identification of A. hastaspicula and A. antarctica, both morphologically and molecularly.

© Wildlife Disease Association 2019
Chloe King, Chin Y. Tay, and Hugh I. Jones "Morphologic Observations and Novel 18S rRNA Sequences of Abbreviata hastaspicula and Abbreviata antarctica from Varanus spp. Lizards in Australia," Journal of Wildlife Diseases 55(1), 149-152, (1 January 2019). https://doi.org/10.7589/2017-11-272
Received: 5 November 2017; Accepted: 6 April 2018; Published: 1 January 2019
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