Our objectives were to establish if the determinant of health model used in the fields of human population and public health could be adapted to wildlife health; if it was applicable to more than one species; and if it reflected how fish and wildlife managers conceptualized health in practice. A conceptual model was developed using a scoping review on fish and wildlife health and resilience coupled with a participatory process with experts on barren ground caribou (Rangifer tarandus groenlandicus) and sockeye salmon (Oncorhynchus nerka) health. Both the literature and experts supported the concept of wildlife health as a cumulative effect involving multiple factors that extend beyond the disease and pathogen focus of many wildlife health studies and legislation. Six themes were associated with fish and wildlife health: 1) the biologic endowment of the individual and population; 2) the animal's social environment; 3) the quality and abundance of the animal's needs for daily living; 4) the abiotic environment in which the animal lives; 5) sources of direct mortality; and 6) changing human expectations. These themes were shared between salmon and caribou and conformed to expert perceptions of health. Determinants of health used in human public health are used for planning, development of policy, and guiding of research. The model we produced may also have use as a wildlife health planning tool to help managers identify health protection priorities and to promote actions across the determinants of health.

Mountain gorillas (Gorilla beringei beringei) are one of the most critically endangered great apes in the world. The most common cause of mountain gorilla morbidity and mortality is trauma (e.g., injury from conspecifics or snare entrapment). We conducted a retrospective case-control study of free-ranging, human-habituated mountain gorillas to evaluate factors associated with snare entrapment and the results of clinical intervention. Data were collected from clinical records on all clinical intervention cases (n=132) in Volcanoes National Park, Rwanda, conducted between 1995–2015. Wildlife veterinarians treated 37 gorillas entrapped in snares and 95 gorillas for other clinical conditions (including trauma and respiratory illness). Multivariate statistical analyses revealed that young gorillas (<8 yr old) were more likely than older gorillas to become snared; that comorbidities delayed times to intervention (≥3 d); and that severity of wounds at the time of intervention were associated with increased risk of lasting impairment (including loss of limb or limb function, or death) within 1 mo after intervention. Our results may influence decisions for gorilla health monitoring and treatment to most effectively conserve this critically endangered species.

Brucellosis, caused by bacteria in the genus Brucella, is an infectious zoonosis affecting animals and humans worldwide. Free-ranging Rocky Mountain elk (Cervus canadensis nelsoni) and bison (Bison bison) in the Greater Yellowstone Ecosystem (areas of southwestern Montana, eastern Idaho, and northwestern Wyoming, US) are the self-sustaining reservoirs of bovine brucellosis (Brucella abortus) and elk are considered the primary source of livestock infections. It has been hypothesized that Brucella-exposed elk might have different physiologic status (pregnancy rates and body condition) and migration behaviors than would healthy elk. Here we tested the effects of brucellosis serologic status on pregnancy rates and winter ingesta free body fat of 100 female elk in southwestern Montana. We also evaluated the effects of serologic status on two characteristics of spring migration behavior, migration types (migrant, mixed migrant, resident, disperser, nomad, and undetermined type) and timing (start and end dates and duration). The migration behaviors were quantified using a model-driven approach based on the relative net squared displacement. We detected a significant difference (P=0.003) in pregnancy rates between seropositive and seronegative elk, with about a 30% drop in seropositive individuals. However, we did not detect differences in body fat between seropositive and seronegative elk or differences in either migration type or timing of spring migration. These results confirmed that the major effect of brucellosis in free-ranging elk is associated with reproduction.

The appearance of West Nile virus (WNV) coincided with declines in California, US bird populations beginning in 2004, and particularly affected corvid populations, including Yellow-billed Magpies (Pica nutalli), an endemic species to California. Our objective was to determine if the timing of the WNV epidemic correlated with changes in the genetic diversity or population structure of magpies. We hypothesized the declines in magpie abundance from WNV would lead to genetic bottlenecks and reduced genetic diversity, but not to changes in population genetic structure. To test these hypotheses, we genetically typed magpie samples collected during the Dead Bird Survey before WNV arrived (2002–04), immediately after WNV arrived in late 2004 (2006–08), and several generations after the onset of the epidemic (2009–11). For each of these three time periods, we tested for genetic bottlenecks, estimated genetic heterozygosity, allelic richness, relatedness, effective population sizes, and genetic structure, with the use of 10 nuclear microsatellite loci. Although there was no evidence for spatial or temporal genetic structure, genetic-diversity estimates were similar or below estimates for endangered corvid species. Measures of genetic diversity were consistent across time periods. In contrast to our expectation, we detected a genetic bottleneck prior to the WNV epidemic, which may have coincided with severe drought conditions in California, increasing human population size in magpie range, and an estimated 33% decrease in population size. We found weak evidence to support a bottleneck after the introduction of WNV in California. Our results suggest the WNV epidemic did not have additional catastrophic effects on the neutral genetic diversity of P. nutalli in the sampled areas. However, because we detected lower heterozygosity in Yellow-billed Magpies than has been reported in closely related endangered species, this species is of conservation concern and should be monitored to detect further population declines or loss of genetic diversity.

We describe a case series of photoreceptor dysplasia with secondary retinal degeneration in juvenile Peregrine Falcons. Six Peregrine Falcons (Falco peregrinus) and three Peregrine Falcon × Prairie Falcon (Falco mexicanus) hybrids had early-life visual deficits. Eight birds had visual defects shortly after hatching, and one bird had visual deficits first noticed at 5 mo of age. Complete ophthalmic examinations were performed in each animal. Eight of the animals had electroretinograms, and nine of the animals had their eyes examined histologically after euthanasia. Ophthalmic examinations did not reveal consistent and potentially blinding abnormalities, including an absence of ophthalmoscopic retinal lesions. Electroretinographic findings included subnormal amplitudes (with rod responses more abnormal than cone responses), with a negative b-wave amplitude occurring in one bird. Histologically, a reduction in the number of photoreceptors was present with numerous degenerative changes to the remaining photoreceptors, including frequent blunting and disorganization of photoreceptor outer segments, decreased numbers of cells in the inner nuclear layer, decreased numbers of ganglion cells, decreased thickness of the nerve fiber layer, and decreased myelinated axons within the optic nerve. Ultrastructurally, only minor cone outer segment changes and occasional phagocytic cells were seen. Results strongly suggested a primary retinopathy, characterized by photoreceptor dysplasia and secondary retinal degeneration with loss of cellular elements throughout the retina. The presence of a similar spectrum of findings in related individuals, the early age of onset, and the relative lack of other environmental, ocular, or systemic abnormalities suggested possible heritability.

Babesia odocoilei, a tick-borne protozoan hemoparasite of white-tailed deer (Odocoileus virginianus), is being increasingly recognized as a cause of disease in captive cervids in North America. Historically endemic in white-tailed deer, the natural wildlife reservoir in the southeastern US, B. odocoilei has been recently associated with hemolytic anemia in captive Eurasian tundra reindeer (Rangifer tarandus tarandus), wapiti (Cervus canadensis), and woodland caribou (Rangifer tarandus caribou) in the northcentral and northeastern US and several Canadian provinces. The emergence of B. odocoilei is likely related to the northward expansion of the range of the tick vector, Ixodes scapularis, and possibly to cervid translocations. Following a disease outbreak in reindeer and wapiti at the Toronto Zoo in Ontario, Canada, we utilized a prospective postmortem survey to investigate the prevalence of B. odocoilei in wild, farmed, and zoo cervids in Ontario (n=270) in 2016–18 by PCR and DNA sequencing of spleen samples. Zoo bovids have been suggested as potential hosts of B. odocoilei in zoos affected by cervid babesiosis, so we also collected postmortem samples from five species of bovids (n=7) at the Toronto Zoo that died or were euthanized during this time. We detected B. odocoilei in 1% (2/142) of farmed red deer (Cervus elaphus) as well as in 3% (1/29) of captive wapiti and 4% (3/68) of wild white-tailed deer. Tissues from all zoo bovids and caribou, zoo and wild moose (Alces alces), and farmed white-tailed deer, wapiti-red deer hybrids, and fallow deer (Dama dama), tested negative for B. odocoilei. No clinical cases of babesiosis were encountered during this study. These findings suggest that white-tailed deer are a potential natural wildlife reservoir for B. odocoilei in Ontario and that red deer and wapiti could serve as more-localized reservoirs.

A histopathologic study of free-ranging hummingbirds found in California, US was performed to identify mortality trends. Tissues from 61 wild hummingbirds representing five native California species collected by the San Diego Zoo from 1996 to 2016 or the Lindsay Wildlife Experience from 2015 to 2017 were histologically examined. Birds were either found dead or moribund at the time of submission or were euthanized due to unresolvable health issues. Long-term rehabilitated birds were excluded from this study. Lesions were sorted by organ, etiology, and gender. The most commonly affected organs were the lung (68%, 40/59), followed by the ingluvies (67%, 34/51) and the liver (54%, 33/61). While some birds had minimal or nonspecific lesions, 23% (14/61) had lesions primarily attributable to trauma, 16% (10/61) had lesions associated with bacteria, fungi, or viruses, 11% (7/61) had parasitic lesions, and 13% (8/61) had multifactorial concurrent processes. Infectious disease lesions included those associated with avian poxvirus, intestinal adenovirus, disseminated aspergillosis, bacterial septicemia, malaria (Haemoproteus spp.), and mycobacteriosis. The most commonly identified parasitic infection was intestinal cestodiasis, for which there was no significant associated intestinal damage, although the large size of these cestodes may have affected digestion. The incidence of traumatic lesions did not vary significantly by sex, age, species, or sampling location. Other significant findings not related to disease or trauma, but not previously documented, were histologic evidence of a gallbladder and the presence of aortic ossification. Our study reported mortality trends at a population level for free-ranging hummingbirds found in California and identified the presence of intestinal adenovirus and two anatomic structures not previously described.

The Bronx River in Bronx, New York, US spans an area of significant human development and has been subject to historic and ongoing industrial contamination. We evaluated the health of freeranging native common snapping turtles (Chelydra serpentina) and nonnative invasive red-eared sliders (Trachemys scripta) in a segment of the Bronx River between May and July 2012. In 18 snapping turtles and nine sliders, complete physical examinations were performed, ectoparasites collected, and blood was analyzed for contaminants (mercury, thallium, cadmium, arsenic, lead, selenium, oxychlordane, alpha-chlordane, dieldrin, DDD, DDE, polychlorinated biphenyls). Complete blood counts and the presence of hemoparasites were determined in 16 snapping turtles and nine sliders. Swabs of the choana and cloaca were screened for ranavirus, adenovirus, herpesvirus, and Mycoplasma spp. by PCR in 39 snapping turtles and 28 sliders. Both turtle species exhibited bioaccumulation of various environmental contaminants, particularly organochlorines and polychlorinated biphenyls. Molecular screening revealed a unique herpesvirus in each species. A Mycoplasma sp. previously isolated from emydid turtles was detected in red-eared sliders while a unique Mycoplasma sp. was identified in common snapping turtles. Ranaviruses and adenoviruses were not detected. Our study established a baseline health assessment to which future data can be compared. Moreover, it served to expand the knowledge and patterns of health markers, environmental contaminants, and microorganisms of freeranging chelonians.

During summer and early fall of 2012, the US experienced the largest outbreak of hemorrhagic disease (HD) on record; deer (both Odocoileus virginianus and Odocoileus hemionus) in 35 states were affected, including many northern states where HD typically does not occur. Epizootic hemorrhagic disease virus (EHDV) was the predominant virus isolated, with serotype 2 (EHDV-2) representing 66% (135/205) of all isolated viruses. Viruses within the EHDV serogroup are genetically similar, but we hypothesized that subtle genetic distinctions between viruses would exist across the geographic range of the outbreak if multiple EHDV-2 strains were responsible. We examined viral relatedness and molecular epidemiology of the outbreak by sequencing the mammalian binding protein (VP2) gene and the insect vector binding protein (VP7) gene of 34 EHDV-2 isolates from 2012 across 21 states. Nucleotide sequences of VP2 had 99.0% pairwise identity; VP7 nucleotide sequences had 99.1% pairwise identity. Very few changes were observed in either protein at the amino acid level. Despite the high genetic similarity between isolates, subtle nucleotide differences existed. Both VP2 and VP7 gene sequences separated into two distinct clades based on patterns of single-nucleotide polymorphisms after phylogenetic analysis. The clades were divided geographically into eastern and western clades, although those divisions were not identical between VP2 and VP7. There was also an association between percent sequence identity and geographic distance between isolates. We concluded that multiple EHDV-2 strains contributed to this outbreak.

During March to August of 2017, hundreds of leopard sharks (Triakis semifasciata) stranded and died on the shores of San Francisco Bay, California, US. Similar mass stranding events occurred in 1967 and 2011, but analysis of those epizootics was incomplete, and no etiology was confirmed. Our investigation of the 2017 epizootic revealed severe meningoencephalitis in stranded sharks, raising suspicion for infection. We pursued a strategy for unbiased pathogen detection using metagenomic next-generation sequencing followed by orthogonal validation and further screening. We showed that the ciliated protozoan pathogen, Miamiensis avidus, was present in the central nervous system of leopard (n=12) and other shark species (n=2) that stranded in San Francisco Bay but was absent in leopard sharks caught elsewhere. This ciliated protozoan has been implicated in devastating outbreaks in teleost marine fish but not in wild elasmobranchs. Our results highlight the benefits of adopting unbiased metagenomic sequencing in the study of wildlife health and disease.

Influenza A viruses (IAVs) are maintained in wild waterbirds and have the potential to infect a broad range of species, including wild mammals. The Arctic Coastal Plain of Alaska supports a diverse suite of species, including waterfowl that are common hosts of IAVs. Mammals co-occur with geese and other migratory waterbirds during the summer breeding season, providing a plausible mechanism for interclass transmission of IAVs. To estimate IAV seroprevalence and identify the subtypes to which geese, loons, Arctic foxes (Vulpes lagopus), caribou (Rangifer tarandus), and polar bears (Ursus maritimus) are potentially exposed, we used a blocking enzyme-linked immunosorbent assay (bELISA) and a hemagglutination inhibition (HI) assay to screen for antibodies to IAVs in samples collected during spring and summer of 2012–16. Apparent IAV seroprevalence using the bELISA was 50.3% in geese (range by species: 46–52.8%), 9% in loons (range by species: 3–20%), and 0.4% in Arctic foxes. We found no evidence for exposure to IAVs in polar bears or caribou by either assay. Among geese, we estimated detection probability from replicate bELISA analyses to be 0.92 and also found good concordance (>85%) between results from bELISA and HI assays, which identified antibodies reactive to H1, H6, and H9 subtype IAVs. In contrast, the HI assay detected antibodies in only one of seven loon samples that were positive by bELISA; that sample had low titers to both H4 and H5 IAV subtypes. Our results provide evidence that a relatively high proportion of waterbirds breeding on the Arctic Coastal Plain are exposed to IAVs, although it is unknown whether such exposure occurs locally or on staging or wintering grounds. In contrast, seroprevalence of IAVs in concomitant Arctic mammals is apparently low.

From 2014 to 2016, we examined the effect of distributing oral rabies vaccine baits at high density (150 baits/km²) in an area of Virginia, US that was naïve to oral rabies vaccination prior to the study. We also compared the effect of baiting at high density in a naïve area to baiting at standard density (75 baits/km²) in an area that had been baited annually for 12 yr. Our results suggested that rabies virus seroconversion in raccoons (Procyon lotor) gradually increased each year under the highdensity bait treatment. However, we did not detect a difference in seroconversion between bait density treatments. Virginia opossums (Didelphis virginiana) were abundant in the study area and were a potentially important nontarget species that competed for oral rabies vaccine baits, but the ratio of opossums to raccoons in this study did not affect rabies virus neutralizing antibody response of the raccoon populations.

A fatal outbreak of sarcoptic mange caused by Sarcoptes scabiei in San Joaquin kit foxes (Vulpes macrotis mutica) in Bakersfield, California, US is causing the once-stable population to decline. Given the fatality of the disease in this already-endangered species experiencing continued population declines, city-wide interventions are underway. To optimize medical management of mange-infested kit foxes, we documented serum biochemistry and hematology values for 11 kit foxes with mange collected from January–May 2015 and compared them to historical data from 18 healthy Bakersfield kit foxes. Results from kit foxes with mange were consistent with chronic illness and inflammation, protein loss, hypoglycemia, and dehydration. These findings contribute to our understanding of this debilitating, multisystemic disease that can progress to death in individuals without intervention and will aid in the treatment and care of rehabilitated individuals.

In Calabria, as in other Italian regions, the wild boar (Sus scrofa) population has increased considerably in the last few years. The presence of this wild ungulate, a huntable species, was detected throughout this region. Wild boars are a host for many endoparasites, some of which are zoonotic. Our aim was to acquire data on the prevalence endoparasites in wild boar in the region of Calabria, Italy, where such information is limited. We used 60 wild boars culled during the 2014–15 hunting season. We used FLOTAC dual techniques for fecal microscopic exams, as well as qualitative and quantitative microscopic examination with a sensitivity level of 2 eggs/g of feces. The research has underlined the remarkable diffusion of gastrointestinal helminths, particularly Ascaris suum, in wild boar of the areas of Sila and Presila in the province of Catanzaro, Calabria.

The West Indian manatee (Trichechus manatus manatus), a subspecies that inhabits coastal areas of Central and South America, has been listed as a vulnerable species because of the rapid decline in its population. Commercially available immunologic reagents specific for sirenians are lacking, limiting the development of sensitive immunodiagnostic assays. We observed the affinity of the microbial proteins A and G to T. m. manatus immunoglobulins. Manatee serum pools were analyzed using enzyme-linked immunosorbent assay (ELISA) to determine the affinity intensity followed by western blotting to confirm the specific binding of proteins A and G to immunoglobulins. The ELISA demonstrated maximum affinity of both proteins until the serum dilution of 1:12,800, with a similar affinity for both proteins. Because both A and G proteins exhibited affinity to manatee immunoglobulins, they can be used to develop sensitive immunodiagnostic assays for this species, contributing to manatee conversation procedures.

West Nile virus (WNV) emerged in the Americas with its introduction in 1999 and now is considered endemic across the continent. In 2002, WNV was detected in Mexico, where its occurrence and mortality are considerably lower compared with the US. However, continuous national surveillance programs in Mexico are nonexistent. Birds are considered the primary hosts and primary geographic dispersers of this pathogen. A total of 200 cloacal and tracheal samples from wild migratory or resident birds were retrospectively analyzed using reverse transcription PCR to detect WNV from birds collected in Mexico from 2008 to 2009. The overall prevalence was 8% (16/200), and positive samples were from Oaxaca, Chiapas, and Tamaulipas in Ruby-throated Hummingbird (Archilochus colubris), Double-crested Cormorant (Phalacrocorax auritus), Ring-billed Gull (Larus delawarensis), and Mourning Dove (Zenaida macroura). Analysis of the partial sequence of the envelope gene from one of the samples from Oaxaca provided evidence that the virus belonged to the WN99 genotype. Taken together, these results demonstrated that WNV circulated in wild birds from northern and southern Mexico during the 2008–09 season, providing further information about the presence of WNV in Mexico.

Anticoagulant rodenticides (ARs) are widely used across North America to control rodent infestations but may cause direct mortality or nonlethal effects when secondarily consumed by raptors. Barn Owls (Tyto alba) are at high risk for secondary consumption because they specialize in rodent prey and often live in human-made structures. We investigated the exposure of Barn Owls in Kentucky, US, to ARs and to dicoumarol, an anticoagulant compound naturally found in certain moldy forages. We tested the liver tissue of 48 Barn Owl carcasses collected during 2012–16. We confirmed exposure to one or more ARs in 33% of the birds examined and detected dicoumarol in 13% of the samples. Rodenticides detected included brodifacoum, coumachlor, and bromadiolone. The prevalence of detected exposure to brodifacoum for after-hatch-year birds (65%) was significantly (P=0.012) higher than hatch-year birds (22%). Brodifacoum was the most commonly detected AR, found in 88% of AR-positive birds. The pesticide registration for this chemical in the US was canceled in 2015 for general consumer products, which likely resulted in a decreasing rate of detected exposure to brodifacoum during our study. We present these results as an example of secondary exposure rates during a period when a pesticide has been restricted and then removed from the consumer market.

Whole blood, serum, and feather samples from 29 Humboldt Penguins (Spheniscus humboldti) at the Punta San Juan Marine Protected Area, Peru, were analyzed for 55 toxic and essential elements by using inductively coupled plasma mass spectrometry. Mercury (Hg) was analyzed by cold vapor atomic fluorescence. Maximum Hg concentrations in serum (0.0056 mg/g), whole blood (0.297 mg/kg), and feathers (1.8 mg/kg dry weight) were at levels generally not considered to cause health impairment. Of the elements analyzed, only eight (aluminum, calcium, iron, Hg, potassium, magnesium, sodium, and zinc) were detected in serum. These elements, plus selenium and titanium, were also quantifiable in whole blood. Feather analysis detected quantifiable values for the elements found in serum, plus arsenic, boron, barium, copper, manganese, and titanium. Results indicate this important breeding population of endangered penguins did not appear to be exposed to environmental elemental contaminants at levels detrimental to health and reproductive success. However, identification of measurable concentrations of toxic elements at low levels underscores the need for continued environmental monitoring, particularly in the face of expanding regional human populations and industrial growth. These results provide important reference data for temporospatial monitoring of this and other penguin populations.

Trypanosoma cruzi is a vector-borne protozoan parasite that infects seven million individuals in Central and South America and is the etiologic agent of Chagas disease. There are increasing reports of endemic transmission within the southern US. Trypanosoma cruzi occurs in wild raccoons and dogs in Oklahoma, but its endemicity in the state is poorly studied. We suspected Mexican free-tailed bats (Tadarida brasiliensis) contributed to the endemicity of T. cruzi in Oklahoma due to their annual migration from Central America to their North American maternity roosts. During the summer of 2017, we sampled 361 Mexican free-tailed bats for T. cruzi at three maternity roosts in Oklahoma. We collected wing tissues, extracted T. cruzi DNA, amplified target DNA by PCR using the primers TCZ1/TCZ2, and observed amplification by gel electrophoresis. One juvenile Mexican free-tailed bat was positive for T. cruzi resulting in a 0.27% prevalence in the 361 sampled bats. Our finding of a wild bat naturally infected with T. cruzi in Oklahoma provided insight on the endemicity of T. cruzi in underrepresented endemic areas. The positive sample was sequenced, confirmed as T. cruzi, and uploaded to GenBank (no. MG869732). Future research will focus on monitoring T. cruzi prevalence in wild bats and insect vectors to better understand the enzootic emergence of this neglected tropical parasite.

The spectacled flying fox (Pteropus conspicillatus) is listed as vulnerable to extinction in Australia. The species' restricted population is in decline, putatively attributed to decreasing habitat, climatic extremes, anthropogenic activities, and more recently, mass mortality events associated with tick paralysis and neonatal cleft palate syndrome. Knowledge of fundamental physiologic parameters of the species is limited. To address this knowledge gap, we sampled 50 wild-caught adult spectacled flying foxes in June (winter) in Far North Queensland, Australia. Hematologic and plasma biochemistry reference ranges were established, and a suite of urine biochemistry analytes were measured. Analyte values were compared within spectacled flying fox sex cohorts and between the spectacled flying fox and the paraphyletic black flying fox (Pteropus alecto). Significant differences in multiple analytes (including erythrocyte, leucocyte, plasma, and urine biochemistry) were found between spectacled flying fox sex cohorts. The majority of spectacled flying fox analyte values did not differ significantly from black flying fox values. Of those analytes that differed between species (erythrocyte, platelet, eosinophil, liver enzyme, and triglyceride levels), the majority were plausibly explained by intraerythrocyte parasite burden and food resource type. Our findings provide baseline data essential to measure and meaningfully interpret flying fox population health in ecologic, conservation, and epidemiologic contexts.

All sea turtle species are listed on the Red List of the International Union for the Conservation of Nature due to multiple threats. Among these, disease is a frequent cause of stranded sea turtles encountered in rehabilitation centers. Since 2013, we found joint swelling in 13 sea turtles belonging to all four sea turtle species submitted to the Kélonia Sea Turtle Observatory of Reunion Island, France. Affected sea turtles presented with lameness, anorexia, and lethargy. Polyarthritis was radiographically confirmed and lesions were characterized by progressive osteolysis of bones surrounding joints. Anterior flippers were affected in all cases and posterior flippers were also involved in some cases. We isolated several bacterial agents from blood and synovial fluid. We attempted a collective treatment with injectable florfenicol, based on sensitivity results, which was continued for 4 wk and then as needed based on radiographic evolution of the lesions. Radiographic stabilization of the lesions occurred in nine of 13 cases. We reviewed environmental conditions and optimized them to minimize stress that could predispose these rehabilitated sea turtles to opportunistic infections. Handling techniques used to move sea turtles were also improved. While we can make no conclusion regarding the cause of polyarthritis in this population of sea turtles, we successfully managed this poorly described problem in a rehabilitation setting.

Philornis is a neotropical genus of muscid fly that interacts with birds and may affect the development and survival of the birds' offspring. Although Philornis is a relatively common parasite, there is a lack of information about Philornis hosts in several parts of the Americas. In this study, two nests of the Rufousfronted Thornbird (Phacellodomus rufifrons) were collected in Pedro Leopoldo, southeast Brazil. The first contained four nestlings of advanced age (about 20 d old) and a recently emerged Philornis torquans female adult fly. The second nest contained three nestlings (less than 7 d old) and several Philornis torquans subcutaneous larvae. One of the nestlings was infested by 53 larvae, which had attacked several parts of its body and caused individual wounds containing 1 to more than 15 larvae. The length of the larvae ranged from 3 to 18 mm and only one was a second instar; the remaining 69 were third instars. The pupal period lasted 9–13 d. In total, 71 larvae were collected from the nest, with nestling parasitism varying from 7 to 53 larvae (mean- 23.7±25.5 larvae/nestling).

The European wild rabbit (Oryctolagus cuniculus) is a key prey species on the Iberian Peninsula, and several predator species that are at risk of extinction are dependent on them as prey. A new rabbit hemorrhagic disease (RHD) virus genotype (GI.2/RHDV2/b) emerged in 2010 and posed a threat to wild rabbit populations. During a survey aimed at investigating RHD epidemiology in wild rabbits, GI.2/RHDV2/b was detected by duplex real-time PCR in carcasses of one Mediterranean pine vole (Microtus duodecimcostatus) and two white-toothed shrews (Crocidura russula). Laboratory New Zealand white rabbits that were challenged with inocula obtained from the liver of the small mammals died showing RHD lesions, confirming the infectiousness of the isolates. Phylogenetic analysis of the VP60 gene nucleotide sequences showed complete homology between the isolates from the two small mammal species and a high degree of similarity, but not complete homology, to GI.2/RHDV2/b sequences from wild rabbits. The GI.2/RHDV2/b genotype has not been reported in species outside the order Lagomorpha.

Rabies is a fatal zoonotic disease of global importance. Rabies virus is shed in the saliva of infected hosts and is primarily transmitted through bite contact. Canine rabies has been eliminated from the US, but wildlife constitutes more than 90% of the reported cases of animal rabies in the US each year. In the US, several wild carnivore species are reservoirs of distinct variants of rabies virus (RV). After decades of apparent absence, the south-central skunk (SCSK) RV variant was detected in Colorado in 2007 and resulted in a large-scale epizootic in striped skunk (Mephitis mephitis) populations in northern Colorado starting in 2012. We attempted isolation of RV from salivary gland tissues from confirmed rabid carnivores, comprising 51 striped skunks and seven other wild and domestic carnivores collected during 2013 through 2015 in northern Colorado. We isolated RV from 84.0% (158/188; 95% confidence interval=78.1–88.6%) of striped skunk and 71% (17/24; 95% confidence interval =51–85%) of other carnivore salivary glands. These data suggested that infected reservoir and vector species were equally likely to shed the SCSK RV variant and posed a secondary transmission risk to humans and other animals.

Leptospirosis is an occupational risk for military personnel and many cases have been reported worldwide. Rodents are the most important maintenance hosts for Leptospira spp. and may infect both animals and humans. To determine the occurrence and identity of pathogenic Leptospira spp. in rodent and shrew populations in German military camps in Afghanistan, we examined 751 animals (Mus musculus, Cricetulus migratorius, Meriones libycus, Rattus tanezumi, Crocidura cf. suaveolens, and Suncus etruscus) from four military camps in Northern Afghanistan from 2009–12. Leptospiral DNA was found in 1.1% of the animals and only in Mus musculus. Partial secY sequencing identified Leptospira borgpetersenii and Leptospira kirschneri as infecting genomospecies. Multilocus sequence typing was successful in the L. borgpetersenii samples, which were identified as sequence type 155. The low prevalence we observed suggested that the exposure risk of military personnel to infectious Leptospira spp. in the region is low.

Wild birds inhabit in a wide variety of environments and can travel great distances. Thus, wild birds can possibly spread antimicrobial resistance along the way, and this may represent a potential public health concern. We characterized antimicrobial resistance in fecal Escherichia coli and Enterococcus faecalis in wild raptors in the southeastern US. Cloacal samples were collected from 118 wild raptors of 17 species from 18 counties in Alabama and 15 counties in Georgia. A total of 112 E. coli and 76 E. faecalis isolates were recovered, and we found significantly more antimicrobial-resistant E. coli (20/112, 18%) than E. faecalis (6/76, 8%; P=0.05). Five antimicrobialresistant genes: blaTEM-1, blaCTX-M-1, tet(M), cmlA, cat, and gyrA, were identified in antimicrobial-resistant E. coli isolates. Five of 13 (38%) ampicillin-resistant E. coli harbored both bla-TEM-1 and blaCTX-M-1 genes, indicating they are extended-spectrum β-lactamase–carrying strains. Both of the tetracycline resistance genes, tet(M) and tet(L), were identified in E. faecalis isolates. Wild raptors seem to be a reservoir host of antimicrobial-resistant E. coli and E. faecalis and may represent a hazard to animal and human health by transmission of these isolates.

Knowledge of animal visitation to locations where species aggregate is valuable for evaluating potential pathways of inter- and intraspecific transmission of infectious diseases. There is no research evaluating the potential of wallows created by invasive wild pigs (Sus scrofa) as locations of transmission of infectious diseases. We monitored wild pig wallows by using trail cameras to determine the frequency with which wild pigs and native vertebrate species visited wallows and to characterize these interactions for their potential for disease transmission. We placed cameras from 20 June 2016 to 21 September 2016 at 16 individual wallows within 10 wallowing sites. Wild pigs of both sexes visited wallows frequently (115 total visits) for varying durations and exhibited wallowing and drinking behavior. We also observed 12 native mammalian and avian wildlife species using wallows at various frequencies (165 total visits). Of particular interest, nine of these species were observed drinking from wallows. Given the high frequency of wild pig visits to wallows and the drinking behavior they and native wildlife species exhibited, these locations might have an important role in transmission of diseases.

We describe an investigation of an outbreak of conjunctivitis in juvenile House Finches (Haemorhous mexicanus) and California Scrub-jays (Aphelocoma californica) at a central California, US wildlife rehabilitation facility. In late May 2015, the facility began admitting juvenile finches, the majority with normal eyes at intake. In June, with juvenile finches already present, the facility admitted juvenile scrub-jays, all with normal eyes at intake. In July, after conjunctivitis was observed in increasing numbers of juvenile finches and scrub-jays, carcasses were submitted for postmortem examination. Histopathology of five finches and three scrub-jays identified lymphocytic infiltrates in the ocular tissues. Conjunctival swabs from 87% (13/15) finches and 33% (4/12) scrub-jays were PCR-positive for Mycoplasma gallisepticum. One finch and two scrub-jays were PCR-positive for Mycoplasma synoviae. Additionally, gene sequencing (16S ribosomal RNA and 16S-23S intergenic spacer region) identified Mycoplasma sturni from 33% (3/9) scrub-jays. This outbreak of conjunctivitis suggested that M. gallisepticum-infected juvenile finches admitted to and maintained in a multispecies nursery likely resulted in transmission within the facility to healthy juvenile finches and scrub-jays. Evidence of other Mycoplasma spp. in finches and scrub-jays indicates that these species are susceptible to infection and may act as carriers. This outbreak highlighted the need for effective triage and biosecurity measures within wildlife rehabilitation facilities.

We tested sera of 24 free-ranging European brown bears (Ursus arctos) from six regions of Slovakia for antibodies to 10 viral agents. We tested sera by an indirect fluorescence antibody test for antibodies to canine distemper virus (CDV), canine coronavirus (CCV), canine parvovirus type 2 (CPV-2), canine adenovirus, canine parainfluenza virus type 2 (CPIV-2), and canine herpesvirus type 1 (CHV-1). We used an enzyme-linked immunosorbent assay for detection of antibodies to hepatitis E virus, bluetongue virus, West Nile virus (WNV), and Aujeszky's disease virus (ADV). We detected antibodies to CDV, CHV-1, CPV-2, CPIV-2, CCV, WNV, and ADV in seven (29%), three (12%), two (8%), two (8%), one (4%), one (4%), and one (4%) bear, respectively. Evidence of exposure of free-ranging European brown bears to CCV and ADV has not been reported.

We report a case in an Italian wolf (Canis lupus italicus) of pantropic canine coronavirus infection, which has previously been detected only in dogs. The wolf was coinfected by canine parvovirus type 2b and canine adenovirus type 2, which highlighted the crucial role of epidemiologic surveys in European wild carnivores.

We report knemidokoptiasis in a wild male Little Spiderhunter (Arachnothera longirostra cinereicollis; family Nectariniidae; order Passeriformes) from Jambi, Sumatra, Indonesia, in September 2017. Microscopic examination of a scraping from its leg lesion revealed the presence of Knemidocoptes jamaicensis as the cause of the condition.

Canine parvovirus (CPV) was detected in three of 136 samples from dead raccoon dogs (Nyctereutes procyonoides) in the Republic of Korea (South Korea) during 2016–17. By sequence and phylogenetic analysis of the complete VP2 gene, the strain belonged to CPV-2 and would be distinct from the previous reported CPV-2a and CPV-2b strains from Korean domestic dogs (Canis lupus familiaris). The results indicated that the CPV strains from raccoon dogs and domestic dogs might be not circulated between wild and domestic carnivores in Korea.

Disease outbreaks and mortalities caused by largemouth bass virus (LMBV) in largemouth bass (Micropterus salmoides) have been reported in the US. Blood and mucus samples tested by PCR to assess the presence of LMBV in largemouth bass in northeastern Mexico were negative, and further monitoring is needed.

The determination of body condition is a fundamental component of an evaluation during postmortem examinations of cetaceans. Three veterinarians experienced in beluga postmortem techniques subjectively evaluated 62 carcasses of beluga whales (Delphinapterus leucas) in duplicate, using a visual analog scale. The result of evaluation and scoring was repeatable in cachectic animals and animals in very good condition but did not capture the continuum of body condition determinations between the obvious visual conditions.

A free-ranging adult Eurasian lynx (Lynx lynx) captured in Switzerland presented with a severe purulent unilateral conjunctivitis. Chlamydia felis was detected in conjunctival swabs by real-time quantitative PCR. Systemic treatment with oxytetracycline and ketoprofen led to complete recovery. Infection with C. felis has not been previously reported in Eurasian lynx.