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9 April 2019 Affinity of Staphylococcal a and Streptococcal G Proteins to West Indian Manatee (Trichechus manatus manatus) Immunoglobulins
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Abstract

The West Indian manatee (Trichechus manatus manatus), a subspecies that inhabits coastal areas of Central and South America, has been listed as a vulnerable species because of the rapid decline in its population. Commercially available immunologic reagents specific for sirenians are lacking, limiting the development of sensitive immunodiagnostic assays. We observed the affinity of the microbial proteins A and G to T. m. manatus immunoglobulins. Manatee serum pools were analyzed using enzyme-linked immunosorbent assay (ELISA) to determine the affinity intensity followed by western blotting to confirm the specific binding of proteins A and G to immunoglobulins. The ELISA demonstrated maximum affinity of both proteins until the serum dilution of 1:12,800, with a similar affinity for both proteins. Because both A and G proteins exhibited affinity to manatee immunoglobulins, they can be used to develop sensitive immunodiagnostic assays for this species, contributing to manatee conversation procedures.

© Wildlife Disease Association 2019
Marcos A. Mendonça, Marcone L. Santos, Thiago D. Barral, Fernanda L. Attademo, Raphael B. Costa, Roberto Meyer, Stella M. Barrouin-Melo, and Ricardo D. Portela "Affinity of Staphylococcal a and Streptococcal G Proteins to West Indian Manatee (Trichechus manatus manatus) Immunoglobulins," Journal of Wildlife Diseases 55(2), 421-424, (9 April 2019). https://doi.org/10.7589/2018-02-061
Received: 27 February 2018; Accepted: 13 June 2018; Published: 9 April 2019
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