The West Indian manatee (Trichechus manatus manatus), a subspecies that inhabits coastal areas of Central and South America, has been listed as a vulnerable species because of the rapid decline in its population. Commercially available immunologic reagents specific for sirenians are lacking, limiting the development of sensitive immunodiagnostic assays. We observed the affinity of the microbial proteins A and G to T. m. manatus immunoglobulins. Manatee serum pools were analyzed using enzyme-linked immunosorbent assay (ELISA) to determine the affinity intensity followed by western blotting to confirm the specific binding of proteins A and G to immunoglobulins. The ELISA demonstrated maximum affinity of both proteins until the serum dilution of 1:12,800, with a similar affinity for both proteins. Because both A and G proteins exhibited affinity to manatee immunoglobulins, they can be used to develop sensitive immunodiagnostic assays for this species, contributing to manatee conversation procedures.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 55 • No. 2