Serum proteins of the California ground squirrel (Spcrmophilus beecheyi) were analyzed by standard electrophoretic methods. Results were presented in terms of total protein, albumin and albumin-globulin (A/G) ratios. Boundaries between globulin fractions were too obscure to warrant analyses of these elements individually.Total protein means differed in squirrels live-trapped at two locations, and live-trapped squirrels had higher total protein levels than squirrels shot. The mean A/G ratios were also significantly different between locations but not between shot and trapped squirrels. Mean total protein and A/G levels in animals with coccidia in the large intestine were the same as from those without coccidia. There was no correlation between adrenal gland weight and total protein or A/G levels.