Esophageal–pharyngeal fluids from 53 free-ranging marsh deer (Blastocerus dichotomus) captured for a research program in the state of Mato Grosso do Sul, Brazil, were assayed for tuberculosis. Total DNA was extracted, amplified by polymerase chain reaction using specific primers for Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. microti, and M. africanum), and observed by agarose gel electrophoresis stained with ethidium bromide. All samples were negative. This, along with necropsy and histopathology data, suggests that these animals are not shedding and probably do not have active disease.
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1 December 2003
TUBERCULOSIS SURVEY OF FREE-RANGING MARSH DEER (BLASTOCERUS DICHOTOMUS) IN BRAZIL
Janaina O. Luna,
Manoel A. A. Santos,
Edison L. Durigon,
João P. Araújo,
José M. B. Duarte
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Journal of Zoo and Wildlife Medicine
Vol. 34 • No. 4
December 2003
Vol. 34 • No. 4
December 2003
Blastocerus dichotomus
marsh deer
Mycobacterium bovis
PCR
TUBERCULOSIS