A 4-mo-old bison (Bison bison) was evaluated and treated at a university veterinary hospital for a cleft defect in the hard and soft palate. Using a mandibular symphysiotomy approach, the palatal defect was repaired with a Z-plasty pattern in the soft palate and mucoperiosteal flaps in the hard palate. A small area of dehiscence in the rostral aspect of the hard palate, and aspiration pneumonia, were complications, but the bison calf recovered with medical management. Even though this surgical procedure has a high potential for complications, the described technique allowed return to normal feeding and resolution of the aspiration pneumonia by 14 mo postsurgery.