Translator Disclaimer
1 December 2011 Attempted in Vitro Maturation and Fertilization of Postmortem Sumatran Rhinoceros (Dicerorhinus sumatrensis) Oocytes
Author Affiliations +
Abstract

A study was conducted opportunistically to evaluate the potential of rescuing immature oocytes from the ovaries of the Sumatran rhinoceros postmortem. Recovered oocytes (n = 30) were placed in maturation culture for 36 hr and inseminated with frozen-thawed homologous spermatozoa. After culture, evaluation of nuclear maturation status revealed that a large number of oocytes were degenerated (n = 21), but nine oocytes were assessed at the germinal vesicle (n = 3), metaphase I (n = 3), and metaphase II (n = 3) stages. Frozen-thawed Sumatran rhinoceros spermatozoa were capable of binding to the zona pellucida of in vitro matured oocytes, but no fertilization or cleavage resulted. In conclusion, relatively large numbers of oocytes can be obtained by ovarian follicular aspiration postmortem in the Sumatran rhinoceros, and some of these oocytes are capable of achieving nuclear maturation in vitro. However, additional studies are required to improve maturation success and achieve fertilization in culture.

American Association of Zoo Veterinarians
Monica A. Stoops, Helen L. Bateman, Mark K. Campbell, and Terri L. Roth "Attempted in Vitro Maturation and Fertilization of Postmortem Sumatran Rhinoceros (Dicerorhinus sumatrensis) Oocytes," Journal of Zoo and Wildlife Medicine 42(4), 723-726, (1 December 2011). https://doi.org/10.1638/2010-0186.1
Received: 16 October 2010; Published: 1 December 2011
JOURNAL ARTICLE
4 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top