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1 March 2013 MOLECULAR DETECTION OF HISTOPLASMA CAPSULATUM IN THE LUNG OF A FREE-RANGING COMMON NOCTULE (NYC-TALUS NOCTULA) FROM FRANCE USING THE Hcp100 GENE
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Abstract

Histoplasma capsulatum is a dimorphic fungus that is widely distributed in the tropical or subtropical areas of the world and infects several mammalian hosts, mainly bats. Infective propagules grow in bat and bird droppings. A specific molecular marker, a highly sensitive fragment of a co-activator protein-coding gene (Hcp100), was used to detect H. capsulatum in lung samples of wild and captive bats from France using a nested polymerase chain reaction. To determine whether bats in France are potential carriers of H. capsulatum, 83 bats were sampled from two regions in France. Sixty-one specimens belonging to the Pteropus rodricensis (n = 45) and Rousettus aegyptiacus (n = 16) species were collected from a zoologic park (La Palmyre, western France). Twenty-two specimens were recovered from the Natural History Museum (Bourges) including the species Plecotus austriacus (n = 1), Pipistrellus pipistrellus (n = 3), and Nyctalus noctula (n = 18). From the lung DNA samples of 83 dead bats, only one sample of an N. noctula bat from Bourges amplified the H. capsulatum Hcp100 marker. The amplified product was sequenced and revealed a high similarity to the G217B H. capsulatum reference strain sequence that was deposited in the GenBank database. This finding suggests that H. capsulatum is an environmental pathogen in France that may infect bats.

American Association of Zoo Veterinarians
Antonio Ernesto González-González, José Antonio Ramírez, Cécile Marie Aliouat-Denis, Christine Demanche, El Moukhtar Aliouat, Eduardo Dei-Cas, Magali Chabé, and Maria Lucia Taylor "MOLECULAR DETECTION OF HISTOPLASMA CAPSULATUM IN THE LUNG OF A FREE-RANGING COMMON NOCTULE (NYC-TALUS NOCTULA) FROM FRANCE USING THE Hcp100 GENE," Journal of Zoo and Wildlife Medicine 44(1), 15-20, (1 March 2013). https://doi.org/10.1638/1042-7260-44.1.15
Received: 24 April 2011; Published: 1 March 2013
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