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1 June 2015 CHARACTERIZATION OF NATURAL EJACULATES AND SPERM CRYOPRESERVATION IN A GOLDEN EAGLE (AQUILA CHRYSAETUS)
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Abstract

This paper describes the sperm characteristics and response to cooling and freezing of naturally ejaculated semen from a captive, adult golden eagle (Aquila chrysaetus) trained to allow sperm recovery via cooperative copulation. A basic spermiogram was prepared, and sperm motility and morphometric variables recorded using a computer-aided system. For sperm storage, the effects of a polyvinylpyrrolidone-based extender were evaluated at 5°C. The same extender was also used in freezing procedures in which glycerol (11%) and dimethylacetamide (6%) were compared as cryoprotectants. The extender preserved sperm viability over storage periods of up to 6 days. Although sperm motility and percentage live sperm values were poorer for frozen–thawed (5.8–14.6% and 44–42%, respectively) than for fresh samples (46.7 and 74.6%, respectively), no differences were seen between the effects of the two cryoprotectants. These results could be of use when attempting to store the sperm of golden eagles and other raptors.

American Association of Zoo Veterinarians
Silvia Villaverde-Morcillo, Rubén García-Sánchez, Cristina Castaño, Eduar Rodríguez, Fernando Gonzalez, Milagros Esteso, and Julián Santiago-Moreno "CHARACTERIZATION OF NATURAL EJACULATES AND SPERM CRYOPRESERVATION IN A GOLDEN EAGLE (AQUILA CHRYSAETUS)," Journal of Zoo and Wildlife Medicine 46(2), 335-338, (1 June 2015). https://doi.org/10.1638/2013-0293R1.1
Received: 10 January 2014; Published: 1 June 2015
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