The evaluation of radiodensity using quantitative computed tomography (QCT) has several indications in animals. It allows for standardization of reference values for bone densitometry and helps in the clinical diagnosis of diseases. Currently, there is a lack of such data in the literature for Boa constrictor constrictor. Therefore, the objective of this study was to estimate reference values for bone density in boas (Boa constrictor constrictor). Computed tomography scans were performed on 10 adult, healthy, free-ranging boas. Results showed mean values of vertebral trabecular bone for free-ranging boas were 1,237.91 ± 54.61 mg/cm³. Because of the difficulty in obtaining clinical and radiographic diagnoses of metabolic bone diseases, especially in a timely manner, densitometry may become a helpful tool in preventive veterinary medicine.