Translator Disclaimer
1 March 2005 QUANTIFICATION OF WEST NILE VIRUS IN VECTOR MOSQUITO SALIVA
Author Affiliations +
Abstract

Saliva was collected from 4 species of mosquitoes intrathoracically inoculated with West Nile virus (WNV). The amount of infectious virus in the saliva was quantified by plaque assay and the number of WNV genomic equivalents (GE) was measured by reverse transcriptase–polymerase chain reaction. Ochlerotatus triseriatus had the greatest mean amount of infectious virus per saliva collection, followed by Aedes albopictus, Culex pipiens, and Cx. quinquefasciatus. The mean GE/saliva collection was also greatest in Oc. triseriatus, followed by Cx. quinquefasciatus, Cx. pipiens, and Ae. albopictus. The variance of log GE/saliva collection for Ae. albopictus was significantly lower than the variance for the other 3 species. This study provides a basis for comparing this component of vector competence and for determining the amounts of virus inoculated into vertebrates in experimental host competence studies.

Leah Colton, Brad J. Biggerstaff, Alicia Johnson, and Roger S. Nasci "QUANTIFICATION OF WEST NILE VIRUS IN VECTOR MOSQUITO SALIVA," Journal of the American Mosquito Control Association 21(1), 49-53, (1 March 2005). https://doi.org/10.2987/8756-971X(2005)21[49:QOWNVI]2.0.CO;2
Published: 1 March 2005
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top