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1 December 2008 A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells
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Abstract

We report that an aqueous solution containing 10−3 M adenosine triphosphate (ATP) and citrate-phosphate-dextrose-adenine (CPDA-1) can effectively replace transfusable human red blood cells in an in vitro Klun and Debboun bioassay system for evaluating chemicals for mosquito feeding-deterrent activity, using either Aedes aegypti or Anopheles stephensi. These species fed with similar avidity through collagen membrane covering aqueous 10−3 M ATP plus CPDA-1 or red blood cells in CPDA-1 supplemented with ATP. In a 2nd experiment, we evaluated the feeding-deterrent activity of N,N-diethyl-3-methylbenzamide and a newly discovered natural product chemical, (-)-isolongifolenone, against these 2 mosquito species. We found that the feeding-deterrent efficacy of the 2 chemicals was similar whether the feeding stimulant was red blood cells supplemented with ATP or ATP alone with CPDA-1. Since the use of human red blood cells in bioassays raises important health and logistic issues, aqueous ATP with CPDA-1 is a reasonable alternative to human blood cells for routine in vitro chemical screening.

Jerome A. Klun, Matthew Kramer, Aijun Zhang, Shifa Wang, and Mustapha Debboun "A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells," Journal of the American Mosquito Control Association 24(4), 508-512, (1 December 2008). https://doi.org/10.2987/08-5755.1
Published: 1 December 2008
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