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1 September 2011 Mosquito Mass Rearing Technology: A Cold-Water Vortex Device for Continuous Unattended Separation of Anopheles arabiensis Pupae from Larvae
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Abstract

In mass rearing of anopheline mosquitoes, pupae are usually separated from larvae on a daily basis to prevent unwanted adult emergence from trays. Depending on the device and species, 2 physical characteristics have most often been used for separation: buoyant density and size. In this report, we describe a system for continuous separation of Anopheles arabiensis larvae from pupae based on the natural difference in buoyant density and behavior between the 2 stages. We determined that temperatures 4–15°C caused neither mortality nor reduction in likelihood of pupation or emergence. Separation improved as temperatures decreased down to 4°C. We devised and demonstrated a 15°C water vortex separator that we anticipate can process approximately 1 million larvae and pupae per hour with a <0.3% pupal contamination rate and which operates unattended.

Fabrizio Balestrino, Jérémie R. L. Gilles, Sharon M. Soliban, Anton Nirschl, Quentin E. Benedict, and Mark Q. Benedict "Mosquito Mass Rearing Technology: A Cold-Water Vortex Device for Continuous Unattended Separation of Anopheles arabiensis Pupae from Larvae," Journal of the American Mosquito Control Association 27(3), (1 September 2011). https://doi.org/10.2987/10-6085.1
Published: 1 September 2011
JOURNAL ARTICLE
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