We report the mosquito species and their distribution records for Ubin Island, Singapore, based on our field collections in February 2010, routine adult and larval surveys from 1993 to 2009 by Singapore operational officers, and accessioned specimens deposited in the Environmental Health Institute, Singapore, and the US National Museum of Natural History, Washington, DC. A total of 25 species and 6 unidentified species belonging to 9 genera (Aedes, Anopheles, Armigeres, Coquillettidia, Culex, Lutzia, Mansonia, Uranotaenia, and Verrallina) were identified and recorded from Ubin Island, based on specimens collected from 1993 to 2010.