A decrease in host-seeking activity in female Aedes albopictus was observed after exposure to a human hand tested in an olfactometer in the laboratory. The increased treatment times resulted in a decreasing response to a human hand. Fatigued/exhausted female mosquitoes after multiple host-seeking activities did prolong the blood engorgement time, compared with untreated nonfatigued mosquitoes. Also, fatigued mosquitoes showed a significant reduction in human host–attacking rates. The mean duration of protection from mosquito bites of human hand treated with 25% deet (N,N-diethyl-3-methylbenzamide) was higher in fatigued than unfatigued mosquitoes.
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