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1 June 2014 Molecular Identification of Aedes triseriatus and Aedes hendersoni by a Novel Duplex Polymerase Chain Reaction Assay
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Abstract

Aedes triseriatus is the principal vector of La Crosse virus (LACv), which is the most common cause of pediatric arboviral encephalitis in North America. Here we report a novel species-specific polymerase chain reaction (PCR) assay that differentially identifies Ae. triseriatus and Ae. hendersoni. Because these 2 sibling species differ in their abilities to transmit LACv, accurate identification is critical for surveillance, research, and control programs. This duplex assay can detect the presence of both species in a single PCR reaction and is therefore simpler and faster than previously reported methods.

2014 by The American Mosquito Control Association, Inc.
Robert Wilson, Robin Harrison, Michael Riles, Gideon Wasserberg, and Brian D. Byrd "Molecular Identification of Aedes triseriatus and Aedes hendersoni by a Novel Duplex Polymerase Chain Reaction Assay," Journal of the American Mosquito Control Association 30(2), 79-82, (1 June 2014). https://doi.org/10.2987/14-6406.1
Published: 1 June 2014
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