Corazonin is a peptidergic neurohormone of insects that has a variety of physiological roles, it is associated with the control of heartbeat, ecdysis behavior initiation, and cuticle coloration. Corazonine (CRZ) sequences are usually highly conserved throughout those insects studied so far. In the present study, we conducted a comprehensive survey of CRZ isoforms in different insect groups using in silico prediction based on publicly accessible transcriptomic databases. A phylogenetic analysis based on CRZ precursors from 23 insect species of six orders suggests that the evolution of insect CRZ precursors might not concord with the traditional taxonomic classification. Comparative analyses of the CRZ peptide sequences shows that CRZ sequence is well conversed in Hemiptera and Lepidoptera, while different CRZ isoforms occur in Diptera, and high diversity of CRZ isoforms is present in Hymenoptera. In order to describe the high diversity of Hymenoptera CRZ, a total of 29 Hymenoptera pro-CRZ sequences were obtained or in silico predicted. It was found that nine different isoforms of CRZ peptides are present in Hymenoptera, of which six novel isoforms, are first reported here. High diversities of CRZ were found in Apoidea and parasitoid wasps, and three modifications were found in members of Apis, Bombus and Diachasma. The present study will be helpful for further investigate the function of highly diverse CRZs in hymenopterans.