Xanthoria calcicola (Teloschistaceae, Ascomycota) still present on bark in Sweden

For almost twenty years Xanthoria calcicola was considered extinct on bark in Sweden. Here, we report X. calcicola growing on bark at 14 localities in Skåne, southernmost Sweden. In total, ca 300 thalli were observed on bark, and the populations vary from 1 to 200 thalli. In all localities except one X. calcicola was also present and more abundant on neighboring substrates made of stone, such as churchyard walls, church walls or tombstones. Preliminary results from fungal ITS data reveal that haplotypes found on bark are always present in the surrounding wall populations. We conclude that trees are suboptimal habitats for X. calcicola and only colonized when in close vicinity of an established wall population. The most obvious threat to epiphytic X. calcicola is the cutting down of host trees.

Almost twenty years ago, a comprehensive survey of the status of the redlisted ('protection-worthy') lichens of southwestern Sweden was published (Arup et al. 1997). This publication quickly became an important baseline for subsequent Swedish red lists (Gärdenfors 2000(Gärdenfors , 2005(Gärdenfors , 2010 as well as further studies of the lichen flora of the region. The characteristic lichen Xanthoria calcicola Oxner, at that time redlisted as Vulnerable (IUCN category 2), is one of the species included in the study (Arup et al. 1997). It was reported to grow on slightly alkaline substrates, mainly on churchyard walls, but also on church walls. It was refound at seven out of 13 earlier known localities (ca 50%), and 12 new localities were discovered (Arup et al. 1997). It was pointed out that there was no modern observation of X. calcicola growing on bark, in spite of several earlier reports between 1939 and 1948 of the species occurring on various deciduous trees (Aesculus hippocastanum, Acer spp., Fraxinus excelsior, Tilia spp. and Ulmus spp.). Arup et al. (1997) postulated that the disappearance of X. calcicola from the suboptimal bark substrate further corroborated its status as being in decline, proba-bly referring to the hypothesis that ecological retardations start with marginal populations (Kawecki 2008).
Xanthoria calcicola belongs to a European southerntemperate element (Almborn 1948, as X. aureola), extending from southern Scandinavia to the Mediterranean, and from eastern Great Britain to Ukraine and the middle East (Wirth 1995, Hitch et al. 2009). In Sweden, the species has a southern distribution in the lowlands of the southernmost regions; Skåne, Gotland, eastern Småland and Halland (Arup et al. 1997, Nordin et al. 2016. The Swedish localities constitute the northern rim of the total geographical distribution of X. calcicola. We made an attempted complete survey of the supposedly rare (included in all Swedish red lists before 2015; ArtDatabanken 2015) churchyard lichen X. calcicola within the south and west parts of Skåne, a total area of ca 5500 km 2 (Fig. 1). Primarily all churchyards and churches, abandoned churchyards, ruin churches and castle ruins were investigated. Xanthoria calcicola was searched for at all habitat types, including trees, at each locality, the number of thalli estimated, and occurrence of apothecia noted (Lindblom and Blom 2009).

Xanthoria calcicola (Teloschistaceae, Ascomycota) still present on bark in Sweden Louise Lindblom and Hans H. Blom
L. Lindblom (louise.lindblom@uib.no),Dept of Natural History,Univ. Museum of Bergen,Univ. of Bergen,Thormøhlensgate 53A,PO box 7800,Norwegian Inst. of Bioeconomy Research,Fana,Norway. For almost twenty years Xanthoria calcicola was considered extinct on bark in Sweden. Here, we report X. calcicola growing on bark at 14 localities in Skåne, southernmost Sweden. In total, ca 300 thalli were observed on bark, and the populations vary from 1 to 200 thalli. In all localities except one X. calcicola was also present and more abundant on neighboring substrates made of stone, such as churchyard walls, church walls or tombstones. Preliminary results from fungal ITS data reveal that haplotypes found on bark are always present in the surrounding wall populations. We conclude that trees are suboptimal habitats for X. calcicola and only colonized when in close vicinity of an established wall population. The most obvious threat to epiphytic X. calcicola is the cutting down of host trees. Table 1. Visited localities where X. calcicola was found on bark, arranged by year of first observation. All localities are churchyards, except Månstorps gavlar (Månstorp gables), which is a castle ruin since 1678. The two rediscoveries of X. calcicola on bark in our study (2010)(2011)(2012)(2013)(2014) are growing on the same tree species as the original observation (1938)(1939). *) total ITS haplotype. **) in our study (2010)(2011)(2012)(2013)(2014). Locality Year of first obs.
Year revisited Substrate tree spp. The populations found on bark vary in size from 1 to ca 200 thalli (Fig. 2-5). All bark populations are small (1-24 thalli), except one (Skegrie churchyard, Fig. 5). Two are rediscoveries at localities where it has previously been collected on bark and 12 are new discoveries. In total, we have observed ca 300 thalli of X. calcicola growing on bark. At six of the previously known localities, including one where it was reported relatively recently (Malmqvist 2000), the species was not found (Table 1). At all localities except one X. calcicola in addition to bark was present on the surrounding churchyard walls. The only exception was Mölleberga churchyard (Fig. 4B), where there were virtually no lichens on the wall, indicating that it had been recently whitewashed (Lindblom and Blom 2010). The wall populations at localities where X. calcicola grows on bark were, on average, larger than other wall populations (1706 ± 2559 versus 489 ±1360 thalli), and a larger proportion of them possessed thalli with apothecia (71.4% versus 50.5%). Thus, localities with tree populations seem to have important source populations on walls in relation to localities with populations on walls only. Seemingly suitable churchyard trees were present at most of the investigated localities, indicating that colonization of X. calcicola on trees is a rare event. The trees harbouring X. calcicola were always growing close to the wall habitat, up to ≤ 5 m (Fig. 3). These results add to the hypothesis that trees are actually suboptimal habitats for X. calcicola. Hence, bark populations are sinks (sensu Dias 1996) sustained by continuous supply of diaspores from the connected source population on walls (sensu Dias 1996).
Heterothallic species require two compatible partners to produce sexual diaspores and, hence, outbreeding is promoted. Interestingly, we have, although extremely rarely, observed thalli of this heterothallic species with apothecia on bark (Table 1, Fig. 4A). Thus, X. calcicola can potentially disperse by ascospores even when growing on a suboptimal substrate as tree bark. However, whether these ascospores are viable, able to resynthesis with a photobiont, and establish the lichen thallus remains to examine.
It is notable that we never observed X. calcicola growing on trees in tree avenues and parks with an environ-   ment closely resembling churchyards. Such habitats are very common in the open and predominantly cultural landscape of Skåne. During our many travels across Skåne before and during our field study we have searched countless random deciduous trees (of the same species as those colonized by X. calcicola) outside churchyards without ever finding X. calcicola. Neither in the extensive field work of Arup et al. (1997) were any tree populations discovered. Observations of young colonizing populations (small thalli) on walls, cemented posts, etc., at distances up to more than 1 km from known source populations, as well as preliminary statistical analyses from our study, indicate that X. calcicola is not strongly delimited by dispersal distances at the scale of our investigation area. We argue that the reason why X. calcicola has not yet been found on trees away from wall populations is a combination of diaspore density dilution by distance from sources and a clearly suboptimal habitat; there are simply so few diaspore hits on such trees that establishment becomes extremely rare.
Preliminary results from fungal ITS data of samples from the localities with populations of X. calcicola (methods following Lindblom and Ekman 2005) reveal that haplotypes found in bark samples are always present in the surrounding wall populations (Table 1). The mo-lecular data suggest that each population of X. calcicola occurring on bark is a subsample of the connected wall population.
Our results support the hypothesis that establishment on trees are dependent of wall populations in close vicinity, continuously bombarding the suboptimal tree habitats with diaspores. The Mölleberga churchyard locality, where the churchyard wall has recently been whitewashed and the wall population are now without X. calcicola  thalli, would be an interesting case study for investigating whether a population on bark can sustain over time without continuous supply of diaspores.
Xanthoria calcicola is no longer redlisted in Sweden, based on the results of our field investigation submitted to the Swedish expert committee for lichens (ArtDatabanken 2015). Clearly, conservation of this beautiful lichen should focus on the churchyard wall populations, addressing the main threats of whitewashing and pressure cleaning.

Conclusions
Xanthoria calcicola in Skåne is more frequently occurring on tree bark than previously assessed.
Trees are suboptimal habitats for X. calcicola and only colonized when in close vicinity of an established wall population.
Even when growing on a suboptimal susbtrate, in this case tree bark, the heterothallic species X. calcicola is able to produce apothecia.
The most obvious threat to epiphytic X. calcicola is the cutting down of the host tree. In addition, thorough cleaning of walls near the trees is a threat, because it could eradicate the potential source population.