Herein I present the description of two new species, Streptanthus medeirosii N.Jensen from the Tehachapi Mountains in Kern County, and S. juneae N.Jensen from the San Bernardino Mountains in San Bernardino County, each described based on phylogenetic, morphological, geographical, and ecological evidence. In order to distinguish S. medeirosii and S. juneae from similar species, I provide diagnoses, a table comparing morphological characters, a taxonomic key, and maps showing their geographic ranges. To enable these comparisons, I also designate lectotypes for two closely related species, S. bernardinus (Greene) Parish and S. campestris S.Watson.