Cold induces a transformation of adipocytes in white adipose tissue (WAT) into ‘beige’ adipocytes with a brown adipose-like phenotype in a process known as ‘browning’. In the present study, cold acclimation was characterized over 49 days of exposure to 5°C (with controls housed at 25°C) in Tupaia belangeri using physiological indices, positron emission tomography-computed tomography (PET/CT) scan, adipose tissue morphology study, and flow cytometry. The results showed that during cold acclimation, body mass and WAT mass and content increased significantly, and there were significant effects of cold exposure on the mean standardized uptake values (SUV) for inguinal, omental, and mesenteric WAT. Adipose tissue morphology showed that the abdominal omental WAT had taken on characteristics of beige adipocytes, including smaller lipid droplets, and expression of uncoupling protein 1 (UCP1). Flow cytometric analysis showed that a new cell population had appeared in WAT by day 49. In the R1 (original) and R2 (new) groups of cells, 69.6% and 95.3% of the cells were positive for UCP1, respectively. All of these results indicate that cold exposure induced WAT browning, which would increase thermogenic properties in T. belangeri under cold acclimation.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.