Translator Disclaimer
29 August 2019 Potential and Pitfalls of the DNA Metabarcoding Analyses for the Dietary Study of the Large Japanese Wood Mouse Apodemus speciosus on Seto Inland Sea Islands
Author Affiliations +
Abstract

We assessed the dietary profile of the large Japanese wood mouse (Apodemus speciosus) inhabiting the islands of the Seto Inland Sea and adjacent areas by DNA metabarcoding analyses of feces with the chloroplast trnL (UAA) P6 loop intron region and the mitochondrial cytochrome c oxidase subunit I gene as DNA-barcoding markers. The results indicated that A. speciosus consumed a variety of food items. Fagaceae (Quercus sp.) and Rosaceae were frequently utilized plant families, and the Lepidoptera (Noctuidae) was the frequently utilized animal family. The acorn-producing Quercus species was found to be a fundamental diet, while Rosaceae might be characteristic in temperate regions. The high dependence on the Noctuidae moths as an animal dietary component implies that A. speciosus may be reducing the pest abundance in orchards. Technical pitfalls surrounding the DNA metabarcoding methods for feces of the small rodent were also discussed.

© The Mammal Society of Japan
Jun J. Sato, Daisuke Kyogoku, Taketo Komura, Chiaki Inamori, Kouhei Maeda, Yasunori Yamaguchi, and Yuji Isagi "Potential and Pitfalls of the DNA Metabarcoding Analyses for the Dietary Study of the Large Japanese Wood Mouse Apodemus speciosus on Seto Inland Sea Islands," Mammal Study 44(4), 1-11, (29 August 2019). https://doi.org/10.3106/ms2018-0067
Received: 13 October 2018; Accepted: 7 June 2019; Published: 29 August 2019
JOURNAL ARTICLE
11 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top