Reference evapotranspiration (ET_o) is often calculated using the Penman-Monteith (FAO 56 PM; Allen et al 1998) method, which requires data on temperature, relative humidity, wind speed, and solar radiation. But in high-mountain environments, such as the Andean páramo, meteorological monitoring is limited and high-quality data are scarce. Therefore, the FAO 56 PM equation can be applied only through the use of an alternative method suggested by the same authors that substitutes estimates for missing data. This study evaluated whether the FAO 56 PM method for estimating missing data can be effectively used for páramo landscapes in the high Andes of southern Ecuador. Our investigation was based on data from 2 automatic weather stations at elevations of 3780 m and 3979 m. We found that using estimated wind speed data has no major effect on calculated ET_o but that if solar radiation data are estimated, ET_o calculations may be erroneous by as much as 24%; if relative humidity data are estimated, the error may be as high as 14%; and if all data except temperature are estimated, errors higher than 30% may result. Our study demonstrates the importance of using high-quality meteorological data for calculating ET_o in the wet páramo landscapes of southern Ecuador.