Songbirds provide an opportunity to explore the neural mechanism underlying the learning and production of complex sound sequences. Imaging the neural structure at single-cell resolution in large volume tissue helps to disclose the functions of neural circuits involved in the song system. Recent advancements in optical clearing techniques may contribute to successful imaging of large-volume samples of songbird brains. These new clearing methods were originally optimized for rodent nervous tissues and previously have not been tested on avian brains. Here we examined whether SeeDB and ClearT, two of the latest optical clearing methods, are suitable for songbird brains, in addition to rodent brains. These two methods were chosen because of the shortness of time required to complete them and because of their compatibility with common lipophilic dye labeling. We applied these methods to brain sections of Bengalese Finches Lonchura striata var. domestica and laboratory rats Rattus norvegicus, then quantified sample transparency as well as expansion rates. The analyses, using both methods, revealed that finch samples were less transparent than rat brain sections. In samples of both species, ClearT was found to enable higher transparency, but caused more expansion, than SeeDB. It is expected that these clearing methods will be of advantage when investigating the anatomical aspects of the neural basis of birdsong.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.