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1 May 2004 Biodistribution Assessment of a Lutetium(III) Texaphyrin Analogue in Tumor-bearing Mice Using NIR Fourier-transform Raman Spectroscopy
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Abstract

The use of near-infrared (NIR)–excited Fourier-transform (FT) Raman spectroscopy as a technique for evaluating the extent of photosensitizer localization in tumor (human pancreatic adenocarcinomas)–bearing mice has been tested using lutetium(III) texaphyrin analogue Lu-T2B2Tex. The complex was injected subcutaneously in the form of three injections given during the course of 3 days. The kinetics of biodistribution were then followed over a time scale of 1–6 days. The NIR-FT-Raman spectra of tissue samples obtained from the xenographic tumor, muscle, heart, brain, liver, spleen, kidney and blood were recorded and used to identify the presence of Lu-T2B2Tex in these tissues. Five Raman sensitizer markers were used to estimate the relative content of Lu-T2B2Tex in tumor at various postinjection times. UV–Visible (Vis) absorption spectroscopic detection of this sensitizer in tissue extracts was applied as a conventional method. Both spectroscopic methods were in good agreement with each other and confirm that Lu-T2B2Tex localizes well in tumor tissue. Maximal drug content was observed 3 days after the final injection. This time delay seems to be optimal for tumor irradiation in photodynamic therapy.

Alla Synytsya, Vladimír Král, Pavel Matejka, Pavla Poučková, Karel Volka, and Jonathan L. Sessler "Biodistribution Assessment of a Lutetium(III) Texaphyrin Analogue in Tumor-bearing Mice Using NIR Fourier-transform Raman Spectroscopy," Photochemistry and Photobiology 79(5), 453-460, (1 May 2004). https://doi.org/10.1562/HE-03-05.1
Received: 3 March 2003; Accepted: 1 February 2004; Published: 1 May 2004
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