The results of a survey of Cardiochilinae and Ichneutinae (Hymenoptera: Braconidae) at Konza Prairie near Manhattan, Kansas are reported. Eleven sites representing prairie and woodland/wetland areas, including gallery forest, were sampled in 2001 and 2005 using Malaise traps and a canopy trap. Selected sites were also sampled haphazardly using pan traps and a sweep net. Six species of Cardiochilinae and two species of Ichneutinae were collected. Cardiochilines were collected at woodland/wetland sites only except a single specimen collected at a prairie site. Both species of Ichneutinae were collected at woodland/wetland sites and prairie sites. Cardiochiles floridanus (Ashmead) (n53) and Cardiochiles minutus (Cresson) (n54) were the most abundant species of Cardiochilinae in 2001 and 2005, respectively. Paroligoneurus newharti Kula, new species, the most abundant species of Ichneutinae in 2001 and only ichneutine collected in 2005, is described. Cardiochiles insculptus Mao and Cardiochiles minutus (Cresson) are reported from Kansas for the first time.