Species of Braconidae from Mount Desert Island and the Schoodic Peninsula area of Acadia National Park (ANP) in Maine are reported based on identification of specimens from a bioblitz conducted at ANP in 2015 in combination with records reported previously. The bioblitz yielded 400 specimens of Braconidae representing 104 species in 46 genera. A list of 175 braconid species is provided. The following species are reported from Maine for the first time: Adelius (Adelius) coloradensis Muesebeck, Alysia (Alysia) lucia Haliday, Aphaereta genevensis Fischer, Chorebus (Chorebus) harringtoni (Ashmead), Ephedrus (Ephedrus) lacertosus (Haliday), Doryctes (Doryctes) macrocaudus Marsh, Leiophron (Euphoriella) incerta (Ashmead), Meteorus politus (Provancher), and Aleiodes (Aleiodes) parasiticus Norton. A new species of Rogadinae (Hymenoptera: Braconidae), Clinocentrus choiradogaster King, Shimbori, and Kula, is described from the Nearctic Region. DNA sequence data for the 28S rDNA expansion region D2, a 618-bp fragment of the barcoding region of cytochrome c oxidase subunit I (COI), and a fragment of cytochrome c oxidase subunit II (COII) are reported for the new species. Diagnostic morphological features for Clinocentrus Haliday and Aleiodes Wesmael (Braconidae: Rogadinae) are discussed given morphological attributes of the new species.