A survey of Lepidoptera reared from seeds and fruits primarily on Barro Colorado Island, Panama, yielded 444 specimens of Tortricidae representing 20 species. Nearly 90% of reared tortricids and 65% of the species are members of the tribe Grapholitini, a group that includes numerous economically important pests of fruit worldwide. We present host records for the following species: Histura panamana Brown, Platynota obliqua Walsingham complex, Platynota subargentea Walsingham, Spinipogon triangularis Brown, Cryptaspasma perseana Gilligan and Brown, Steblopotamia streblopa (Meyrick), Cydia pyraspis (Meyrick) complex, Eriosocia guttifera (Meyrick), Riculorampha ancyloides Rota and Brown, Grapholita mabea Razowski, Ricula croceus Brown, Ricula lacistema Brown, Ricula sp. 1, Ricula sp. 2, Talponia sp. 1, Talponia sp. 2, and four unidentified Grapholitini. In a comparison of studies focused on fruit- and seed-feeding insects in Thailand, Panama, and Kenya, Grapholitini represented 73% (in Thailand) to 90% (in Panama) of the total number of reared specimens of Tortricidae, and 45% (in Kenya) to 65% (in Panama) of the total number of tortricid species. However, a similar survey in Papua New Guinea produced considerably different results, with Grapholitini representing 46% of the tortricid specimens and only 20% of the species.