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1 January 2003 Influence of Environmental pH on G2-Phase Arrest Caused by Ionizing Radiation
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Abstract

Park, H. J., Lee, S. H., Chung, H., Rhee, Y. H., Lim, B. U., Ha, S. W., Griffin, R. J., Lee, H. S., Song, C. W. and Choi, E.;thK. Influence of Environmental pH on G2-Phase Arrest Caused by Ionizing Radiation. Radiat. Res. 159, 86–93 (2003).

We investigated the effects of an acidic environment on the G2/M-phase arrest, apoptosis, clonogenic death, and changes in cyclin B1-CDC2 kinase activity caused by a 4-Gy irradiation in RKO.C human colorectal cancer cells in vitro. The time to reach peak G2/M-phase arrest after irradiation was delayed in pH 6.6 medium compared to that in pH 7.5 medium. Furthermore, the radiation-induced G2/M-phase arrest decayed more slowly in pH 6.6 medium than in pH 7.5 medium. Finally, there was less radiation-induced apoptosis and clonogenic cell death in pH 6.6 medium than in pH 7.5 medium. It appeared that the prolongation of G2-phase arrest after irradiation in the acidic environment allowed for greater repair of radiation-induced DNA damage, thereby decreasing the radiation-induced cell death. The prolongation of G2-phase arrest after irradiation in the acidic pH environment appeared to be related at least in part to a prolongation of the phosphorylation of CDC2, which inhibited cyclin B1-CDC2 kinase activity.

Heon Joo Park, Sang Hwa Lee, HyunSook Chung, Yun Hee Rhee, Byung Uk Lim, Sung Whan Ha, Robert J. Griffin, Hyung Sik Lee, Chang Won Song, and Eun Kyung Choi "Influence of Environmental pH on G2-Phase Arrest Caused by Ionizing Radiation," Radiation Research 159(1), 86-93, (1 January 2003). https://doi.org/10.1667/0033-7587(2003)159[0086:IOEPOG]2.0.CO;2
Received: 8 January 2002; Accepted: 1 September 2002; Published: 1 January 2003
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