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1 July 2003 Genetic Damage Induced by In Vitro Irradiation of Human G0 Lymphocytes with Low-Energy Protons (28 keV/μm): HPRT Mutations and Chromosome Aberrations
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Abstract

Mognato, M., Bortoletto, E., Ferraro, P., Baggio, L., Cherubini, R., Canova, S., Russo, A. and Celotti, L. Genetic Damage Induced by In Vitro Irradiation of Human G0 Lymphocytes with Low-Energy Protons (28 keV/μm): HPRT Mutations and Chromosome Aberrations. Radiat. Res. 160, 52–60 (2003).

Cell survival, mutations and chromosomal effects were studied in primary human lymphocytes exposed in G0 phase to a proton beam with an incident energy of 0.88 MeV (incident LET of 28 keV/μm) in the dose range 0.125–2 Gy. The curves for survival and mutations at the hypoxanthine-guanine phosphoribosyl transferase locus were obtained by fitting the experimental data to linear and linear-quadratic equations, respectively. In the dose interval 0–1.5 Gy, the α parameters of the curves were 0.42/Gy and 3.6 × 10−6 mutants/Gy, respectively. The mutation types at the HPRT locus were analyzed by multiplex-PCR in 94 irradiated and 41 nonirradiated clones derived from T lymphocytes from five healthy donors. All clones showed a normal multiplex-PCR pattern and were classified as point mutations. Chromosome aberration data were fitted as a linear function of dose (α = 0.62 aberrations per cell Gy−1). By irradiating G0 lymphocytes from a single subject with 28 keV/μm protons and γ rays, an RBE of 6.07 was obtained for chromosome aberrations. An overinvolvement of chromosome 9 relative to chromosome 7 was found in chromosome breaks after chromosome painting analysis.

Maddalena Mognato, Elisabetta Bortoletto, Paola Ferraro, Laura Baggio, Roberto Cherubini, Sabrina Canova, Antonella Russo, and Lucia Celotti "Genetic Damage Induced by In Vitro Irradiation of Human G0 Lymphocytes with Low-Energy Protons (28 keV/μm): HPRT Mutations and Chromosome Aberrations," Radiation Research 160(1), (1 July 2003). https://doi.org/10.1667/0033-7587(2003)160[0052:GDIBIV]2.0.CO;2
Received: 4 September 2002; Accepted: 1 January 2003; Published: 1 July 2003
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