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1 July 2003 Identification of Two Differentially Expressed Mitochondrial Genes in a Methotrexate-Resistant Chinese Hamster Cell Strain Derived from V79 Cells Using RNA Fingerprinting by Arbitrary Primed Polymerase Chain Reaction
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Abstract

Chaudhuri, K., Banerjee, R., Pandit, B., Mukherjee, A., Das, S., Sengupta, S., Roychoudury, S. and Bhattacharyya, N. P. Identification of Two Differentially Expressed Mitochondrial Genes in a Methotrexate-Resistant Chinese Hamster Cell Strain Derived from V79 Cells Using RNA Fingerprinting by Arbitrary Primed Polymerase Chain Reaction. Radiat. Res. 160, 77–85 (2003).

To identify genes that are differentially expressed in a methotrexate (MTX)-resistant cell strain designated as M5 that exhibits resistance to γ radiation and a number of chemotherapeutic drugs compared to the parental Chinese hamster V79 cells, we used RNA fingerprinting by arbitrary primed polymerase chain reaction (RAP-PCR). By comparative analysis, we identified six differentially expressed transcripts that were cloned and sequenced. Two of these partial cDNA clones showed high homology to the mitochondrial genes NADH dehydrogenase subunit 1 and subunit 4. The steady-state mRNA level of both the NADH dehydrogenase subunits was about twofold higher in the M5 cell strain compared to V79 cells. Moreover, the expression of both the subunits decreased in γ-irradiated Chinese hamster V79 cells. Cytochrome oxidase, another enzyme of the mitochondrial electron transport chain encoded in the mitochondrial genome, was also found to be overexpressed in M5 cells. All three genes are under the control of the same promoter. However, no amplification of DNA was observed. These data indicate that the alterations in mitochondrial gene expression may be involved in the recovery of irradiated cells, which may arise from transcriptional modulation of the mitochondria from the nucleus.

Keya Chaudhuri, Ramanuj Banerjee, Bhaswati Pandit, Aparna Mukherjee, Soumita Das, Sharmila Sengupta, Susanta Roychoudury, and Nitai P. Bhattacharyya "Identification of Two Differentially Expressed Mitochondrial Genes in a Methotrexate-Resistant Chinese Hamster Cell Strain Derived from V79 Cells Using RNA Fingerprinting by Arbitrary Primed Polymerase Chain Reaction," Radiation Research 160(1), (1 July 2003). https://doi.org/10.1667/RR3004
Received: 4 September 2001; Accepted: 1 November 2002; Published: 1 July 2003
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