Translator Disclaimer
7 March 2016 Dependence of Gold Nanoparticle Radiosensitization on Functionalizing Layer Thickness
Author Affiliations +

Gold nanoparticles functionalized with polyethylene glycol of different chain lengths are used to determine the influence of the capping layer thickness on the radiosensitizing effect of the particles. The size variations in organic coating, built up with polyethylene glycol polymers of molecular weight 1–20 kDa, allow an evaluation of the decrease in dose enhancement percentages caused by the gold nanoparticles at different radial distances from their surface. With localized eradication of malignant cells as a primary focus, radiosensitization is most effective after internalization in the nucleus. For this reason, we performed controlled radiation experiments, with doses up to 20 Gy and particle diameters in a range of 5–30 nm, and studied the relaxation pattern of supercoiled DNA. Subsequent gel electrophoresis of the suspensions was performed to evaluate the molecular damage and consecutively quantify the gold nanoparticle sensitization. In conclusion, on average up to 58.4% of the radiosensitizing efficiency was lost when the radial dimensions of the functionalizing layer were increased from 4.1 to 15.3 nm. These results serve as an experimental supplement for biophysical simulations and demonstrate the influence of an important parameter in the development of nanomaterials for targeted therapies in cancer radiotherapy.

Cedric Spaas, Rüveyda Dok, Olivier Deschaume, Bert De Roo, Mattias Vervaele, Jin Won Seo, Carmen Bartic, Peter Hoet, Frank Van den Heuvel, Sandra Nuyts, and Jean-Pierre Locquet "Dependence of Gold Nanoparticle Radiosensitization on Functionalizing Layer Thickness," Radiation Research 185(4), (7 March 2016).
Received: 22 July 2015; Accepted: 1 November 2015; Published: 7 March 2016

Get copyright permission
Back to Top