Translator Disclaimer
7 December 2016 The Withers Archive: Online Availability of H. Rodney Withers' Data
Author Affiliations +

Dr. H. Rodney Withers (1932–2015) (11, 2) was one of the preeminent radiation biologists of his time, beginning in the 1960s and spanning more than 4 decades. He was a translational researcher who recognized the importance of stem cells and who asked questions about biological systems relevant to clinical practice. Dr. Withers was the embodiment of a translational physician scientist well before the concept became popular. His preclinical mouse data provided clinical radiation therapists rational guidelines for making treatment choices easily understood and highly accessible. “The 4 R's of Radiotherapy” coined by him summarized the key mechanisms governing tumor and normal tissue responses to fractionated radiotherapy while providing the biological bases to alter dose fractionation schemes for clinical benefit. In many ways, providing the data based rationale for exploiting these responses sums up his legacy for the radiation sciences. Repair of sublethal damage preferentially spares late responding normal tissues. Reassortment of surviving cells within the division cycle resensitizes rapidly proliferating cells. Reoxygenation of hypoxic cells during fractionated treatment radiosensitizes tumors. Repopulation of surviving clonogenic cells during a course of treatment is a hazard best minimized in the case of tumors and encouraged for acute responding normal tissues.

Prior to his death from Parkinson's disease in 2015, his long-time colleagues Kathy Mason and Howard Thames discussed with him about making his immense data base of normal tissue responses available to the worldwide scientific community by converting his handwritten lab notebooks into searchable digitized data files. Dr. Withers wholeheartedly agreed and over a span of 4 years, tens of thousands of animal observations were digitized by Nancy Hunter into the “Withers Archive”. Some of these results have never been analyzed or published, and as we and former collaborators enter retirement it would have been impossible for future investigators to gain access to his data. For this reason, we have digitized the contents of the lab notebooks. It was Rod's hope and ours that this data will have interest to newer scientists in the fields of radiation oncology and biology and their future use of this data base will continue to benefit cancer patients now and in the future. In this note we wish to explain the mechanism we have set up to make the raw data contained in the “Withers Archive” available to researchers online.

Organization of the Data

The data have been stored in 23 Microsoft Excel files whose contents are briefly described in Table 1. The files are grouped either by tissue (e.g., jejunum) or the nature of the end point (e.g., acute skin reaction). The experimental design is briefly described. Relevant publications (when available) are cited for each file.

TABLE 1

i0033-7587-186-6-659-t01.eps

Each of the 23 Excel files contains two sheets. The first sheet, titled “Explanatory”, sets out the details of the experiments, including radiation source, strain of mice, fractionation schedule, etc. In addition, the details of the data formatting are explained. The second sheet, titled “Data”, contains the digitized data.

A researcher who is interested in obtaining portions of this data would begin by identifying what parts are wanted, according to the terminology used in Table 1. Afterward, please ensure that this terminology is used when contacting staff at the Center for Radiation Oncology Research (CROR) at MD Anderson Cancer Center (as explained in more detail below).

Two-Step Exchange of Emails to Obtain the Data

First step.The requestor identifies the tissues/end points of interest from Table 1. The requestor then sends an email to the staff at CROR (CRORadmin@mdanderson.org) requesting more detailed information. The staff at CROR will then match the requested tissues/end points (using the terminology from Table 1) to various “synopses”, which set out the contents of the Excel files in detail. The appropriate synopses will be emailed to the requestor.

Second step.The requestor uses the synopses to identify specific Excel files that match their needs, and emails that list of Excel files back to CRORadmin@mdanderson.org. The requested files are then emailed back to the requestor.

Acknowledgment of Re-publication

If the data provided as described in this note are used in any future publications, it is requested that the following acknowledgment be cited: “Data used in this study were obtained from the Withers Archive, the digitization of results from the research groups of H.R. Withers and his colleagues were provided by the Center for Radiation Oncology Research at the MD Anderson Cancer Center”.

Acknowledgements

REFERENCES

1.

McBride WH, Mason KA, Peters LJ, Thames HD. Dr. H. Rodney withers (1932 - 2015). Int J Radiat Biol 2015; 91:459–61. Google Scholar

2.

McBride WH, Mason KA, Peters LJ, Thames HD. In memoriam: H. Rodney Withers (21 September 1932 – 25 February 2015). Radiat Res 2015; 183:586–8. Google Scholar

3.

Thames HD Jr, Withers R, Mason KA, Reid BO. Dose-survival characteristics of mouse jejunal crypt cells. Int J Radiat Oncol Biol Phys 1981; 11:1591–7. Google Scholar

4.

Thames HD Jr, Withers HR. Test of equal effect per fraction and estimation of initial clonogen number in microcolony assays of survival after fractionated irradiation. Br J Radiol 1980; 53:1071–7. Google Scholar

5.

Withers HR. Cell renewal system concepts and the radiation response. In: Frontiers of Radiation Therapy Oncology. Karger, Basel & University Park Press; 6:93–107, 1972. Google Scholar

6.

Mason KA, Thames HD, Ochran TG, Ruifrok AC, Janjan N. Comparison of continuous and pulsed low dose rate brachytherapy: biological equivalence in vivo. Int J Radiat Oncol Biol Phys 1994; 28:667–71. Google Scholar

7.

Mason KA, Gillin MT, Mohan R, Cox JD. Preclinical biologic assessment of proton beam relative biologic effectiveness at Proton Therapy Center Houston. Int J Radiat Oncol Biol Phys 2007; 68:968–70. Google Scholar

8.

Withers HR, Mason K, Reid BO, Dubravsky N, Barkley HT Jr, Brown BW, Smathers JB. Response of mouse intestine to neutrons and gamma rays in relation to dose fractionation and division cycle. Cancer 1974; 34:39–47. Google Scholar

9.

Withers HR, Chu AM, Mason KA, Reid BO, Barkley HT Jr, Smathers JB. Response of jejunal mucosa to fractionated doses of neutrons or gamma-rays. Eur J Cancer 1974: 249–52. Google Scholar

10.

Hall EJ, Withers HR, Geraci JP, Meyn RE, Rasey J, Todd P, Sheline GE. Radiobiological intercomparisons of fast neutron beams used for therapy in Japan and the United States. Int J Radiat Oncol Biol Phys 1979; 5:227–33. Google Scholar

11.

Meyn RE, Peters LJ, Mills MD, Moyers MF, Fields RS, Withers HR, Mason KA. Radiobiological aspects of electron beams. The role of high energy electrons in the treatment of cancer. In: Frontiers of Radiation Therapy Oncology. Vol. 25. In: Vaeth JM, Meyer JL, editors. Karger: Basel, 1991: p. 53–60. Google Scholar

12.

Mason KA, Withers HR, McBride WH, Davis CA, Smathers JB. Comparison of the gastrointestional syndrome after total-body or total-abdominal irradiation. Radiat Res 1989; 117:480–8. Google Scholar

13.

Withers HR, Reid Bo, Hussey DH. Response of mouse jejunum to multifraction radiation. Int J Radiat Oncol Biol Phys 1975; 1:41–52. Google Scholar

14.

Sheu T, Molkentine J, Transtrum MK, Buchholz TA, Withers HR, Thames HD, Mason KA. Use of the LQ model with large fraction sizes results in underestimation of isoeffect doses. Radiother Oncol 2013; 109:21–5. Google Scholar

15.

Wiedenmann N, Valdecanas D, Hunter N, Hyde S, Buchholz TA, Milas L, Mason KA. 130-nm albumin-bound paclitaxel enhances tumor radiocurability and therapeutic gain. Clin Cancer Res 2007; 13:1868–74. Google Scholar

16.

Tucker SL, Withers HR, Mason KA, Thames HD Jr. A dose-surviving fraction curve for mouse colonic mucosa. Eur J Cancer Clin Oncol 1983; 19:433–7. Google Scholar

17.

Withers HR, Mason KA. The kinetics of recovery in irradiated colonic mucosa of the mouse. Cancer 1974; 34:suppl:896-903. Google Scholar

18.

Withers HR, Hunter N, Barkley HT Jr, Reid BO. Radiation survival and regeneration characteristics of spermatogenic stem cells of mouse testis. Radiat Res 1974; 57:88–103. Google Scholar

19.

Suzuki N, Withers HR. Exponential decrease during aging and random lifetime of mouse spermatogonial stem cells. Science 1978; 202:1214–5. Google Scholar

20.

Meistrich ML, Hunter NR, Suzuki N, Trostle PK, Withers HR. Gradual regeneration of mouse testicular stem cells after exposure to ionizing radiation. Radiat Res 1978; 74:349–62. Google Scholar

21.

Thames HD Jr, Withers HR. Test of equal effect per fraction and estimation of initial clonogen number in microcolony assays of survival after fractionated irradiation. Br J Radiol 1980; 53:1071–7. Google Scholar

22.

Masuda K, Hunter N, Stone HB, Withers HR. Leg contracture in mice after single and multifractionated 137Cs exposure. Int J Radiat Oncol Biol Phys 1987; 13:1209–15. Google Scholar

23.

Masuda K, Matsuura K, Withers HR, Hunter N. Age dependency of response of the mouse skin to single and multifractionated gamma irradiation. Radiother Oncol 1986; 7:147–53. Google Scholar

24.

Ruifrok AC, Mason KA, Hunter N, Thames HD. Changes in the radiation sensitivity of mouse skin during fractionated and prolonged treatments. Radiat Res 1994; 139:334–43. Google Scholar

25.

Masuda K, Hunter N, Withers HR. Late effect in mouse skin following single and multifractionated irradiation. Int J Radiat Oncol Biol Phys 1980; 6:1539–44. Google Scholar

26.

Masuda K, Hunter N, Withers HR. Early skin shrinkage in mice after single and multifractionated gamma-ray exposure. J Radiat Res 1982; 23:313–27. Google Scholar

27.

Masuda K, Matsuura K, Withers HR, Hunter N. Response of previously irradiated mouse skin to a second course of irradiation: early skin reaction and skin shrinkage. Int J Radiat Oncol Biol Phys 1986; 12:1645–51. Google Scholar

28.

Masuda K, Hunter N, Withers HR, Matsuura K. Radiosensitivity of irradiated mouse skin to a second course of single and multifractionated irradiation. I. Early skin reaction. Radiat Med 1983; 1:85–8. Google Scholar

29.

Jingu K, Masuda K, Withers HR, Hunter N. Radiosensitivity of pre-irradiated mouse skin to second courses of single and multi-fractionated irradiation—skin shrinkage. Radiother Oncol 1989; 14:143–50. Google Scholar

30.

Masuda K, Reid BO, Hunter N, Withers HR. Bone growth retardation induced by single and multifractionated irradiation. Radiother Oncol 1990; 18:137–45. Google Scholar

31.

Tucker SL, Thames HD, Brown BW, Mason KA, Hunter NR, Withers HR. Direct analyses of in vivo colony survival after single and fractionated doses of radiation. Int J Radiat Biol 1991; 59:777–95. Google Scholar

32.

Withers HR, Mason KA, Thames HD Jr. Late radiation response of kidney assayed by tubule-cell survival. Br J Radiol 1986; 59:587–95. Google Scholar

33.

Mason KA, Withers HR, Chiang CS. Late effects of radiation on the lumbar spine cord of guinea pigs: re-treatment tolerance. Int J Radiat Oncol Biol Phys 993; 26:643–8. Google Scholar

34.

Taylor JM, Mason KA, Vegesna V, Withers HR. A non-parametric method of reconstructing single-dose survival curves from multi-fraction experiments. Int J Radiat Biol 1998; 74:583–93. Google Scholar

35.

Kogelnik HD, Withers HR. Radiobiological considerations in multifraction irradiation. Radiol Clin (Basel) 1978; 47:362–9. Google Scholar
©2016 by Radiation Research Society.
H. D. Thames, N. R. Hunter, and K. A. Mason "The Withers Archive: Online Availability of H. Rodney Withers' Data," Radiation Research 186(6), 659-661, (7 December 2016). https://doi.org/10.1667/RR4638.1
Received: 3 October 2016; Published: 7 December 2016
JOURNAL ARTICLE
3 PAGES


SHARE
ARTICLE IMPACT
Back to Top