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13 December 2016 Exosome-Mediated Telomere Instability in Human Breast Epithelial Cancer Cells after X Irradiation
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In directly irradiating cells, telomere metabolism is altered and similar effects have been observed in nontargeted cells. Exosomes and their cargo play dominant roles in communicating radiation-induced bystander effects with end points related to DNA damage. Here we report novel evidence that exosomes are also responsible for inducing telomere-related bystander effects. Breast epithelial cancer cells were exposed to either 2 Gy X rays, or exposed to irradiated cell conditioned media (ICCM), or exosomes purified from ICCM. Compared to control cells, telomerase activity decreased in the 2 Gy irradiated cells and both bystander samples after one population doubling. At the first population doubling, telomere length was shorter in the 2 Gy irradiated sample but not in the bystander samples. By 24 population doublings telomerase activity recovered to control levels in all samples; however, the 2 Gy irradiated sample continued to demonstrate short telomeres and both bystander samples acquired shorter telomeres. RNase treatment of exosomes prevented the bystander effects on telomerase and telomere length that were observed at 1 population doubling and 24 population doublings, respectively. Thermal denaturation by boiling eliminated the reduction of telomere length in bystander samples, suggesting that the protein fraction of exosomes also contributes to the telomeric effect. RNase treatment plus boiling abrogated all telomere-related effects in directly irradiated and bystander cell populations. These findings suggest that both proteins and RNAs of exosomes can induce alterations in telomeric metabolism, which can instigate genomic instability in epithelial cancer cells after X-ray irradiation.

©2017 by Radiation Research Society.
Ammar H. J. Al-Mayah, Scott J. Bright, Debbie A. Bowler, Predrag Slijepcevic, Edwin Goodwin, and Munira A. Kadhim "Exosome-Mediated Telomere Instability in Human Breast Epithelial Cancer Cells after X Irradiation," Radiation Research 187(1), 98-106, (13 December 2016).
Received: 14 July 2015; Accepted: 1 September 2016; Published: 13 December 2016

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