We describe the advertisement call and calling perch of Ikakogi tayrona, a glassfrog species endemic to the Sierra Nevada de Santa Marta (Colombia), based on 85 calls from 16 individuals. The advertisement call consists of 3–4 “beep” notes with amplitude modulation. Mean note duration was 0.024 ± 0.005 s, with notes separated by silent intervals of 0.085 ± 0.017 s, mean call duration was 0.269 ± 0.031 s, and the dominant frequency of the whole call was 2.713 ± 0.102 kHz, being inversely related with body size of the signaler. The advertisement call of I. tayrona is clearly distinguished from the call of other glassfrogs and species of Allophrynidae, the sister clade of Centrolenidae. Males called frequently from the underside of leaves. The height of the calling perch ranged from 51–250 cm above the ground. Since I. tayrona is an early-diverging lineage of glassfrogs, our data offer important baseline information to understand the evolutionary biology of auditory signals and calling behavior in centrolenid frogs.
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