Translator Disclaimer
30 September 2018 Evaluation of Genotoxic and Mutagenic Effects of Glyphosate Roundup Original® in Dendropsophus minutus Peters, 1872 Tadpoles
Author Affiliations +
Abstract

The herbicide glyphosate is widely used on Brazilian crops and commonly found in water bodies. Despite its usefulness in controlling pests and increasing agricultural production, improper use of this environmental contaminant might accelerate the decline of species, especially frogs. The aim of this study was to evaluate the genotoxic and mutagenic effects of glyphosate Roundup Original® in tadpoles of Dendropsophus minutus by comet assay and micronucleus test. Fourteen egg masses were collected in two permanent ponds in Chapada dos Veadeiros National Park in Alto Paraíso de Goiás, Brazil. Acute exposure was conducted with 180 tadpoles maintained for 99 d in a storage tank until developmental stages 26–31. During acclimation, tadpoles were distributed among 36 tanks and exposed to the following concentrations of the active ingredient (ai) glyphosate Roundup Original®: 0.28 mg ai/L, 1.0 mg ai/L, 2.0 mg ai/L, 4.0 mg ai/L of, negative control (0 mg ai/L), and positive control (10 mg ai/L mitomycin C). After 96 h of exposure, the tadpoles were euthanized with 5% benzocaine and submitted to comet assay and micronucleus test. In the comet assay, a significant increase in DNA damage was observed in response to the 0.28 mg ai/L concentration for tail length, % DNA in the tail, and Olive tail moment. There was no dose-response effect at other concentrations. For the micronucleus test, we did not find a statistically significant increase in micronuclei in erythrocytes of D. minutus after 96 h of exposure to glyphosate. Therefore, in the present study we found that tadpoles of D. minutus are extremely sensitive to low concentrations of glyphosate, demonstrating the importance of amphibian tadpoles in studies of genotoxicity and mutagenicity.

© 2018 Brazilian Society of Herpetology
Wanessa Fernandes Carvalho, Wanessa Fernandes Carvalho, Fernanda Craveiro Franco, Fernanda Craveiro Franco, Fernanda Ribeiro Godoy, Fernanda Ribeiro Godoy, Daiany Folador, Daiany Folador, Juliana Boaventura Avelar, Juliana Boaventura Avelar, Fausto Nomura, Fausto Nomura, Aparecido Divino da Cruz, Aparecido Divino da Cruz, Simone Maria Teixeira de Sabóia-Morais, Simone Maria Teixeira de Sabóia-Morais, Rogério Pereira Bastos, Rogério Pereira Bastos, Daniela de Melo e Silva, Daniela de Melo e Silva, "Evaluation of Genotoxic and Mutagenic Effects of Glyphosate Roundup Original® in Dendropsophus minutus Peters, 1872 Tadpoles," South American Journal of Herpetology 13(3), (30 September 2018). https://doi.org/10.2994/SAJH-D-17-00016.1 . Submission: Received: 13 March 2017; Accepted: 18 January 2018
Received: 13 March 2017; Accepted: 18 January 2018; Published: 30 September 2018
JOURNAL ARTICLE
10 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top